Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 36607..37129 Replicon plasmid pNT1N25-tetX4
Accession NZ_CP075485
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI28_RS22995 Protein ID WP_000220560.1
Coordinates 36848..37129 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI28_RS22990 Protein ID WP_000121743.1
Coordinates 36607..36858 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS22965 32278..32559 + 282 WP_001185482.1 hypothetical protein -
IGI28_RS22970 32584..33561 + 978 WP_024245156.1 hypothetical protein -
IGI28_RS22975 33558..33914 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI28_RS22980 34051..34497 - 447 Protein_40 hypothetical protein -
IGI28_RS22985 34537..35373 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI28_RS22990 36607..36858 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI28_RS22995 36848..37129 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI28_RS23000 37204..37901 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
IGI28_RS23005 38047..38304 + 258 WP_105278499.1 hypothetical protein -
IGI28_RS23010 38341..39309 - 969 WP_077783444.1 IS5 family transposase -
IGI28_RS23015 39344..39619 + 276 Protein_47 peptidoglycan DD-metalloendopeptidase family protein -
IGI28_RS23020 39653..39850 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / tet(A) / blaTEM-1B / tet(M) / qnrS1 / mef(B) / sul3 / dfrA5 / tet(X4) - 1..115741 115741
- flank IS/Tn - - 38341..39264 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203798 WP_000220560.1 NZ_CP075485:36848-37129 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203798 NZ_CP100336:1650343-1650525 [Streptococcus suis]
ATGCCTATGACACAAAAAGAAATGGTTAAGCTACTTACCGCTCATGGTTGGACAAAGACCAAAGGCGGTAAGGGGTCACA
CGTTAAACTTGAAAAAGCTGGCGAGCGACCTATAACCATTCCCCACGGTGAAATTAACAAATATACTGAACGGGGTATCA
AAAAGCAAGCAGGGCTGTTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203798 WP_000121743.1 NZ_CP075485:36607-36858 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203798 NZ_CP100336:1650564-1651016 [Streptococcus suis]
ATGCTTGTCACTTATCCAGCTTTATTTTATTATGACGATTCAGACGGAGCTAACGCCCCTTATTTTGTCACTTTCCCAGA
CTTTGAACATTCAGCAACTCAAGGCGAAGATATGGCAGACGCTATGGCAATGGCTAGTGATTGGTTAGGTATTCATCTAG
CTGATTATATTGAGAATGGGCGAGATATTCCCACTCCTGCCCCTATTAACACTCTATCTTTAGCAAATAATAACCCTTTC
CGTGATGATGAGGATATAGAGCTAGTTTATGACCCTAGCAAGTCTTTTGTATCTATGGTTATGGTTGACGTTGCTGAGTA
TCTAGGCAGTCAAGAGCCTGTAAAGAAAACACTAACTATTCCACGTTGGGCGGATACTTTAGGGCGTGAGTTAGGGCTAA
ATTTCTCACAGACCTTAACAGATGCTATTGCAGATAAGAAAATTCATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References