Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4086201..4086796 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag IGI28_RS20005 Protein ID WP_000239579.1
Coordinates 4086201..4086551 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag IGI28_RS20010 Protein ID WP_001223208.1
Coordinates 4086545..4086796 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS19985 (4081234) 4081234..4082736 - 1503 WP_000205791.1 sugar ABC transporter ATP-binding protein -
IGI28_RS19990 (4083109) 4083109..4084089 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
IGI28_RS19995 (4084325) 4084325..4085281 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
IGI28_RS20000 (4085591) 4085591..4086121 + 531 WP_000055072.1 inorganic diphosphatase -
IGI28_RS20005 (4086201) 4086201..4086551 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
IGI28_RS20010 (4086545) 4086545..4086796 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
IGI28_RS20015 (4087009) 4087009..4087350 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
IGI28_RS20020 (4087353) 4087353..4091132 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4083109..4084089 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T203795 WP_000239579.1 NZ_CP075484:c4086551-4086201 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T203795 NZ_CP100336:c969882-969616 [Streptococcus suis]
ATGAAGACCTGTTATAAACTCGTACCGACCAGTCGTTTTATTAAACAGTTGAAAAAGTTGGACAAATTTACCCAAAAACA
GATTACCAACTACCTGTCCAGTCATGTGACTGACAACCCTAGACAATACGGAAAAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGCTACCGTATCGGCAATTACAGGGTTATCGTCAGCATTGAAGATGACAAATTGATTGTGACAGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT203795 WP_001223208.1 NZ_CP075484:c4086796-4086545 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT203795 NZ_CP100336:c970117-969866 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAGTACGATGCGCGTGTGGCAGACGTTGCTTGGGCAG
AATACCAAGCAGACTTGGCGAATAGAATTGAGCCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGGTTGACGGATGAA
GACCTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References