Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3479009..3479846 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IGI28_RS17180 Protein ID WP_000227784.1
Coordinates 3479304..3479846 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IGI28_RS17175 Protein ID WP_001297137.1
Coordinates 3479009..3479320 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS17150 (3474029) 3474029..3474976 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
IGI28_RS17155 (3474998) 3474998..3476989 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IGI28_RS17160 (3476979) 3476979..3477593 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IGI28_RS17165 (3477593) 3477593..3477922 + 330 WP_025649657.1 cytochrome o ubiquinol oxidase subunit IV -
IGI28_RS17170 (3477934) 3477934..3478824 + 891 WP_000971336.1 heme o synthase -
IGI28_RS17175 (3479009) 3479009..3479320 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IGI28_RS17180 (3479304) 3479304..3479846 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IGI28_RS17185 (3479902) 3479902..3480837 - 936 WP_001297127.1 tetratricopeptide repeat protein -
IGI28_RS17190 (3481245) 3481245..3482609 + 1365 WP_001000978.1 MFS transporter -
IGI28_RS17195 (3482737) 3482737..3483228 - 492 WP_001138904.1 nucleotide binding protein YajQ -
IGI28_RS17200 (3483396) 3483396..3484307 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T203793 WP_000227784.1 NZ_CP075484:3479304-3479846 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T203793 NZ_CP100334:c2080395-2080138 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACATTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT203793 WP_001297137.1 NZ_CP075484:3479009-3479320 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT203793 NZ_CP100334:c2080657-2080397 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References