Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1362641..1363266 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IGI28_RS07035 Protein ID WP_000911329.1
Coordinates 1362868..1363266 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IGI28_RS07030 Protein ID WP_000450524.1
Coordinates 1362641..1362868 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS07005 (1358443) 1358443..1358913 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IGI28_RS07010 (1358913) 1358913..1359485 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IGI28_RS07015 (1359631) 1359631..1360509 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IGI28_RS07020 (1360526) 1360526..1361560 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IGI28_RS07025 (1361773) 1361773..1362486 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IGI28_RS07030 (1362641) 1362641..1362868 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IGI28_RS07035 (1362868) 1362868..1363266 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
IGI28_RS07040 (1363413) 1363413..1364276 + 864 WP_001267498.1 neutral zinc metallopeptidase -
IGI28_RS07045 (1364291) 1364291..1366306 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
IGI28_RS07050 (1366380) 1366380..1367078 + 699 WP_000679812.1 esterase -
IGI28_RS07055 (1367188) 1367188..1367388 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T203779 WP_000911329.1 NZ_CP075484:1362868-1363266 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T203779 NZ_CP100330:1321418-1322263 [Streptococcus suis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTCATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TTTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCCAAACTGAAGAAAGAAGACATTGACTTCATTCAGTTGAGAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGTAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAAGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTCTCATAACAGTTCTCATTTCTATAAATTCTGATTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT203779 WP_000450524.1 NZ_CP075484:1362641-1362868 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT203779 NZ_CP100330:1320831-1321421 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGAATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAATATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTTTGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References