Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 926053..926707 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IGI28_RS05015 Protein ID WP_000244772.1
Coordinates 926300..926707 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IGI28_RS05010 Protein ID WP_000354046.1
Coordinates 926053..926319 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS04985 (921341) 921341..922084 + 744 WP_001436959.1 SDR family oxidoreductase -
IGI28_RS04990 (922141) 922141..923574 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
IGI28_RS04995 (923619) 923619..923930 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IGI28_RS05000 (924094) 924094..924753 + 660 WP_000250274.1 hemolysin III family protein -
IGI28_RS05005 (924830) 924830..925810 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IGI28_RS05010 (926053) 926053..926319 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IGI28_RS05015 (926300) 926300..926707 + 408 WP_000244772.1 protein YgfX Toxin
IGI28_RS05020 (926747) 926747..927268 - 522 WP_001055874.1 flavodoxin FldB -
IGI28_RS05025 (927380) 927380..928276 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IGI28_RS05030 (928301) 928301..929011 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IGI28_RS05035 (929017) 929017..930750 + 1734 WP_000813213.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T203777 WP_000244772.1 NZ_CP075484:926300-926707 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203777 NZ_CP100330:c969884-969618 [Streptococcus suis]
ATGAAGACCTGTTATAAACTCGTACCGACCAGTCGTTTTATTAAACAGTTGAAAAAGTTGGACAAATTTACCCAAAAACA
GATTACCAACTACCTGTCCAGTCATGTGACTGACAACCCTAGACAATACGGAAAAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGCTACCGTATCGGCAATTACAGGGTTATCGTCAGCATTGAAGATGACAAATTGATTGTGACAGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203777 WP_000354046.1 NZ_CP075484:926053-926319 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203777 NZ_CP100330:c970119-969868 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAGTACGATGCGCGTGTGGCAGACGTTGCTTGGGCAG
AATACCAAGCAGACTTGGCGAATAGAATTGAGCCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGGTTGACGGATGAA
GACCTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References