Detailed information of TA system TA203776

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 716847..717682
• Replicon: chromosome
• Accession: NZ_CP075484
• Organism: Escherichia coli strain NT1N25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: IGI28_RS03950
• Protein ID: WP_000854821.1
• Coordinates: 717305..717682 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IGI28_RS03945
• Protein ID: WP_072972366.1
• Coordinates: 716847..717215 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI28_RS03915 (714021)	714021..714335	-	315	Protein_673	antirestriction protein	-
IGI28_RS03920 (714426)	714426..715223	-	798	WP_193114909.1	DUF932 domain-containing protein	-
IGI28_RS03925 (715189)	715189..715359	+	171	Protein_675	antirestriction protein	-
IGI28_RS03930 (715374)	715374..715850	+	477	WP_193114910.1	RadC family protein	-
IGI28_RS03935 (715913)	715913..716134	+	222	WP_000692353.1	DUF987 domain-containing protein	-
IGI28_RS03940 (716153)	716153..716797	+	645	WP_059239987.1	hypothetical protein	-
IGI28_RS03945 (716847)	716847..717215	+	369	WP_072972366.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IGI28_RS03950 (717305)	717305..717682	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
IGI28_RS03955 (717679)	717679..718167	+	489	WP_059239990.1	DUF5983 family protein	-
IGI28_RS03960 (718179)	718179..718376	+	198	WP_000839248.1	DUF957 domain-containing protein	-
IGI28_RS03965 (718461)	718461..719306	+	846	WP_049038750.1	DUF4942 domain-containing protein	-
IGI28_RS03975 (719606)	719606..720112	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
IGI28_RS03980 (720190)	720190..722031	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T203776 WP_000854821.1 NZ_CP075484:717305-717682 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T203776 NZ_CP100330:563108-563368 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13623.38 Da
• Isoelectric Point: 6.2044

>AT203776 WP_072972366.1 NZ_CP075484:716847-717215 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRVGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT203776 NZ_CP100330:562885-563106 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Structures

Toxin

• AlphaFold DB: A0A7W4PV54