Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4107815..4108312 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI30_RS19780 Protein ID WP_001173343.1
Coordinates 4107815..4107988 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI30_RS19785 Protein ID WP_000398619.1
Coordinates 4108016..4108312 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS19770 (4104480) 4104480..4105937 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IGI30_RS19775 (4106174) 4106174..4107691 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI30_RS19780 (4107815) 4107815..4107988 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI30_RS19785 (4108016) 4108016..4108312 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI30_RS19790 (4108539) 4108539..4108811 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IGI30_RS19795 (4108823) 4108823..4109053 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IGI30_RS19800 (4109234) 4109234..4110865 + 1632 WP_001216477.1 sensor histidine kinase -
IGI30_RS19805 (4110862) 4110862..4111581 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203765 WP_001173343.1 NZ_CP075480:c4107988-4107815 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203765 NZ_CP100326:c972195-971929 [Streptococcus suis]
ATGAAGACCTGTTATAAACTCGTACCGACCAGTCGTTTTATTAAACAGTTGAAAAAGTTGGACAAATTTACCCAAAAACA
GATTACCAACTACCTGTCCAGTCATGTGACTGACAACCCTAGACAATACGGAAAAGCCTTAACTGCCAATCGTTCTGGCC
AATGGCGCTACCGTATCGGCAATTACAGGGTTATCGTCAGCATTGAAGATGACAAATTGATTGTGACAGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203765 WP_000398619.1 NZ_CP075480:c4108312-4108016 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203765 NZ_CP100326:c972430-972179 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAGTACGATGCGCGTGTGGCAGACGTTGCTTGGGCAG
AATACCAAGCAGACTTGGCGAATAGAATTGAGCCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGGTTGACGGATGAA
GACCTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References