Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3566439..3567273 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2X7CAN1
Locus tag IGI30_RS17170 Protein ID WP_039025883.1
Coordinates 3566439..3566816 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2X5LBZ2
Locus tag IGI30_RS17175 Protein ID WP_039025882.1
Coordinates 3566905..3567273 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS17145 (3562410) 3562410..3562562 - 153 WP_000788776.1 hypothetical protein -
IGI30_RS17150 (3563380) 3563380..3564699 + 1320 WP_000144688.1 site-specific integrase -
IGI30_RS17155 (3564792) 3564792..3565640 - 849 WP_039025884.1 DUF4942 domain-containing protein -
IGI30_RS17160 (3565737) 3565737..3565934 - 198 WP_000772024.1 DUF957 domain-containing protein -
IGI30_RS17165 (3565954) 3565954..3566442 - 489 Protein_3377 DUF5983 family protein -
IGI30_RS17170 (3566439) 3566439..3566816 - 378 WP_039025883.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
IGI30_RS17175 (3566905) 3566905..3567273 - 369 WP_039025882.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IGI30_RS17180 (3567323) 3567323..3567967 - 645 WP_039025881.1 hypothetical protein -
IGI30_RS17185 (3567982) 3567982..3568203 - 222 WP_000692312.1 DUF987 domain-containing protein -
IGI30_RS17190 (3568272) 3568272..3568748 - 477 WP_001186742.1 RadC family protein -
IGI30_RS17195 (3568763) 3568763..3569248 - 486 WP_000214317.1 antirestriction protein -
IGI30_RS17200 (3569339) 3569339..3570157 - 819 WP_001234702.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14176.22 Da        Isoelectric Point: 7.8523

>T203762 WP_039025883.1 NZ_CP075480:c3566816-3566439 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Download         Length: 378 bp

>T203762 NZ_CP100313:17676-18092 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13792.52 Da        Isoelectric Point: 5.9582

>AT203762 WP_039025882.1 NZ_CP075480:c3567273-3566905 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT203762 NZ_CP100313:17449-17679 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X7CAN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2X5LBZ2

References