Detailed information of TA system TA203760

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2752329..2753163
• Replicon: chromosome
• Accession: NZ_CP075480
• Organism: Escherichia coli strain NT1N31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: W0S379
• Locus tag: IGI30_RS13365
• Protein ID: WP_000854808.1
• Coordinates: 2752329..2752706 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IGI30_RS13370
• Protein ID: WP_193112592.1
• Coordinates: 2752795..2753163 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI30_RS13325 (2747336)	2747336..2747827	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
IGI30_RS13330 (2747929)	2747929..2748483	-	555	WP_001001921.1	molecular chaperone YcdY	-
IGI30_RS13335 (2748507)	2748507..2749244	-	738	WP_000283667.1	zinc-binding phosphatase	-
IGI30_RS13340 (2749299)	2749299..2750237	-	939	Protein_2626	glyoxylate/hydroxypyruvate reductase GhrA	-
IGI30_RS13350 (2750708)	2750708..2751550	-	843	WP_193107732.1	DUF4942 domain-containing protein	-
IGI30_RS13355 (2751635)	2751635..2751832	-	198	WP_072643848.1	DUF957 domain-containing protein	-
IGI30_RS13360 (2751844)	2751844..2752332	-	489	WP_039022338.1	DUF5983 family protein	-
IGI30_RS13365 (2752329)	2752329..2752706	-	378	WP_000854808.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
IGI30_RS13370 (2752795)	2752795..2753163	-	369	WP_193112592.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IGI30_RS13375 (2753213)	2753213..2753857	-	645	WP_072643847.1	antitoxin of toxin-antitoxin stability system	-
IGI30_RS13380 (2753876)	2753876..2754097	-	222	WP_000692312.1	DUF987 domain-containing protein	-
IGI30_RS13385 (2754166)	2754166..2754642	-	477	WP_072645454.1	RadC family protein	-
IGI30_RS13390 (2754657)	2754657..2755142	-	486	WP_000214317.1	antirestriction protein	-
IGI30_RS13395 (2755233)	2755233..2756051	-	819	WP_001234702.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgG / manB / manC / fimH / fimG / fimF / fimD	2744645..2813048	68403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13909.93 Da
• Isoelectric Point: 7.9085

>T203760 WP_000854808.1 NZ_CP075480:c2752706-2752329 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T203760 NZ_CP100312:c4865530-4864613 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13631.50 Da
• Isoelectric Point: 7.0261

>AT203760 WP_193112592.1 NZ_CP075480:c2753163-2752795 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVAPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHIVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT203760 NZ_CP100312:c4866294-4865536 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTTGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: W0S379