Detailed information of TA system TA020375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5304054..5304649
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: ECs_5203
• Protein ID: NP_313230.2
• Coordinates: 5304335..5304649 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: ECs_5202
• Protein ID: NP_313229.2
• Coordinates: 5304054..5304305 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_5199	5299719..5303498	+	3780	NP_313226.1	hypothetical protein	-
ECs_5200	5303501..5303842	+	342	NP_313227.1	oxidoreductase	-
ECs_5202	5304054..5304305	+	252	NP_313229.2	antitoxin of the ChpBS toxin-antitoxin system	Antitoxin
ECs_5203	5304335..5304649	+	315	NP_313230.2	toxin of the ChpB-ChpS toxin-antitoxin system	Toxin
ECs_5204	5304729..5305259	-	531	NP_313231.1	inorganic pyrophosphatase	-
ECs_5205	5305569..5306525	+	957	NP_313232.1	galactofuranose ABC transporter periplasmic binding protein	-
ECs_5206	5306665..5308167	+	1503	NP_313233.1	sugar ABC transporter ATPase	-
ECs_5207	5308181..5309203	+	1023	NP_313234.2	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-102)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11090.80 Da
• Isoelectric Point: 5.5667

>T20375 NP_313230.2 NC_002695:5304335-5304649 [Escherichia coli O157:H7 str. Sakai]
VLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARL
AKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 315 bp

>T20375 NC_002695:5304335-5304649 [Escherichia coli O157:H7 str. Sakai]
GTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGGTGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGC
CTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTC
CTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTTAATCAGGTGCGGATGATGGATCTACGCGCCCGGCTG
GCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT20375 NP_313229.2 NC_002695:5304054-5304305 [Escherichia coli O157:H7 str. Sakai]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT20375 NC_002695:5304054-5304305 [Escherichia coli O157:H7 str. Sakai]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA