Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 854204..854858 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IGI30_RS04185 Protein ID WP_000244777.1
Coordinates 854451..854858 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IGI30_RS04180 Protein ID WP_000354046.1
Coordinates 854204..854470 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS04155 (849373) 849373..850116 + 744 WP_000951962.1 SDR family oxidoreductase -
IGI30_RS04160 (850173) 850173..851606 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IGI30_RS04165 (851651) 851651..851962 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IGI30_RS04170 (852126) 852126..852785 + 660 WP_000250274.1 hemolysin III family protein -
IGI30_RS04175 (852981) 852981..853961 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IGI30_RS04180 (854204) 854204..854470 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IGI30_RS04185 (854451) 854451..854858 + 408 WP_000244777.1 protein YgfX Toxin
IGI30_RS04190 (854898) 854898..855419 - 522 WP_001055874.1 flavodoxin FldB -
IGI30_RS04195 (855531) 855531..856427 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IGI30_RS04200 (856452) 856452..857162 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IGI30_RS04205 (857168) 857168..858901 + 1734 WP_032237580.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T203747 WP_000244777.1 NZ_CP075480:854451-854858 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203747 NZ_CP100308:c5187861-5187478 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203747 WP_000354046.1 NZ_CP075480:854204-854470 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203747 NZ_CP100308:c5188103-5187861 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References