Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 698485..699320 Replicon chromosome
Accession NZ_CP075480
Organism Escherichia coli strain NT1N31

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W4PV54
Locus tag IGI30_RS03375 Protein ID WP_000854821.1
Coordinates 698943..699320 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A4Q1HU91
Locus tag IGI30_RS03370 Protein ID WP_053287563.1
Coordinates 698485..698853 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI30_RS03340 (694452) 694452..695324 + 873 WP_053292456.1 GTPase family protein -
IGI30_RS03345 (695493) 695493..695903 + 411 Protein_659 autotransporter domain-containing protein -
IGI30_RS03350 (696230) 696230..697048 + 819 WP_096200128.1 DUF932 domain-containing protein -
IGI30_RS03355 (697139) 697139..697624 + 486 WP_000214317.1 antirestriction protein -
IGI30_RS03360 (697639) 697639..698115 + 477 WP_001186747.1 RadC family protein -
IGI30_RS03365 (698184) 698184..698405 + 222 WP_000692309.1 DUF987 domain-containing protein -
IGI30_RS03370 (698485) 698485..698853 + 369 WP_053287563.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IGI30_RS03375 (698943) 698943..699320 + 378 WP_000854821.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
IGI30_RS03380 (699317) 699317..699514 + 198 Protein_666 DUF5983 family protein -
IGI30_RS03385 (699542) 699542..699739 + 198 WP_088209831.1 DUF957 domain-containing protein -
IGI30_RS03390 (699824) 699824..700669 + 846 WP_063101552.1 DUF4942 domain-containing protein -
IGI30_RS03400 (700968) 700968..701474 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
IGI30_RS03405 (701553) 701553..703394 - 1842 WP_000437375.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13930.95 Da        Isoelectric Point: 8.5163

>T203745 WP_000854821.1 NZ_CP075480:698943-699320 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T203745 NZ_CP100308:c4699173-4698889 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13663.36 Da        Isoelectric Point: 6.4768

>AT203745 WP_053287563.1 NZ_CP075480:698485-698853 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT203745 NZ_CP100308:c4699405-4699163 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W4PV54

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1HU91

References