Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 24050..24572 Replicon plasmid pNT1W25-tetX4
Accession NZ_CP075479
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI33_RS23020 Protein ID WP_000220560.1
Coordinates 24291..24572 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI33_RS23015 Protein ID WP_000121743.1
Coordinates 24050..24301 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS22990 19721..20002 + 282 WP_001185482.1 hypothetical protein -
IGI33_RS22995 20027..21004 + 978 WP_024245156.1 hypothetical protein -
IGI33_RS23000 21001..21357 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI33_RS23005 21494..21940 - 447 Protein_26 hypothetical protein -
IGI33_RS23010 21980..22816 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI33_RS23015 24050..24301 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI33_RS23020 24291..24572 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI33_RS23025 24647..25344 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
IGI33_RS23030 25490..25747 + 258 WP_105278499.1 hypothetical protein -
IGI33_RS23035 25784..26752 - 969 WP_077783444.1 IS5 family transposase -
IGI33_RS23040 26772..27062 + 291 WP_073503549.1 peptidoglycan DD-metalloendopeptidase family protein -
IGI33_RS23045 27096..27293 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1A / tet(M) / qnrS1 / dfrA5 / sul3 / mef(B) / tet(X4) / floR / tet(A) - 1..102128 102128
- flank IS/Tn - - 25784..26752 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203742 WP_000220560.1 NZ_CP075479:24291-24572 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203742 NZ_CP100308:3974919-3975137 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203742 WP_000121743.1 NZ_CP075479:24050-24301 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203742 NZ_CP100308:3974518-3974892 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References