Detailed information of TA system TA020374

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 5207161..5207658
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: ECs_5775
• Protein ID: YP_009502672.1
• Coordinates: 5207485..5207658 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: ECs_5110
• Protein ID: NP_313137.1
• Coordinates: 5207161..5207457 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_5106	5203892..5204611	-	720	NP_313133.1	two-component regulatory system response regulator DcuR	-
ECs_5107	5204608..5206239	-	1632	NP_313134.1	two-component system sensor histidine kinase DcuS	-
ECs_5108	5206420..5206650	+	231	NP_313135.1	hypothetical protein	-
ECs_5109	5206662..5206934	+	273	NP_313136.1	hypothetical protein	-
ECs_5110	5207161..5207457	+	297	NP_313137.1	antitoxin of GhoTS toxin-antitoxin system	Antitoxin
ECs_5775	5207485..5207658	+	174	YP_009502672.1	toxin of GhoTS toxin-antitoxin pair	Toxin
ECs_5111	5207777..5209294	-	1518	NP_313138.1	lysine tRNA synthetase	-
ECs_5112	5209531..5210988	-	1458	NP_313139.1	dipeptide and tripeptide permease D	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T20374 YP_009502672.1 NC_002695:5207485-5207658 [Escherichia coli O157:H7 str. Sakai]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T20374 NC_002695:5207485-5207658 [Escherichia coli O157:H7 str. Sakai]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT20374 NP_313137.1 NC_002695:5207161-5207457 [Escherichia coli O157:H7 str. Sakai]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT20374 NC_002695:5207161-5207457 [Escherichia coli O157:H7 str. Sakai]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG