Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4088838..4089433 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag IGI33_RS19595 Protein ID WP_000239579.1
Coordinates 4088838..4089188 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag IGI33_RS19600 Protein ID WP_001223208.1
Coordinates 4089182..4089433 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS19575 (4083871) 4083871..4085373 - 1503 WP_000205791.1 sugar ABC transporter ATP-binding protein -
IGI33_RS19580 (4085746) 4085746..4086726 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
IGI33_RS19585 (4086962) 4086962..4087918 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
IGI33_RS19590 (4088228) 4088228..4088758 + 531 WP_000055072.1 inorganic diphosphatase -
IGI33_RS19595 (4088838) 4088838..4089188 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
IGI33_RS19600 (4089182) 4089182..4089433 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
IGI33_RS19605 (4089646) 4089646..4089987 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
IGI33_RS19610 (4089990) 4089990..4093769 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4085746..4086726 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T203737 WP_000239579.1 NZ_CP075477:c4089188-4088838 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T203737 NZ_CP100308:350686-351033 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT203737 WP_001223208.1 NZ_CP075477:c4089433-4089182 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT203737 NZ_CP100308:351033-351332 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References