Detailed information of TA system TA203735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3481646..3482483
• Replicon: chromosome
• Accession: NZ_CP075477
• Organism: Escherichia coli strain NT1W25

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IGI33_RS16770
• Protein ID: WP_000227784.1
• Coordinates: 3481941..3482483 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IGI33_RS16765
• Protein ID: WP_001297137.1
• Coordinates: 3481646..3481957 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI33_RS16740 (3476666)	3476666..3477613	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IGI33_RS16745 (3477635)	3477635..3479626	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IGI33_RS16750 (3479616)	3479616..3480230	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IGI33_RS16755 (3480230)	3480230..3480559	+	330	WP_025649657.1	cytochrome o ubiquinol oxidase subunit IV	-
IGI33_RS16760 (3480571)	3480571..3481461	+	891	WP_000971336.1	heme o synthase	-
IGI33_RS16765 (3481646)	3481646..3481957	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IGI33_RS16770 (3481941)	3481941..3482483	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IGI33_RS16775 (3482539)	3482539..3483474	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
IGI33_RS16780 (3483882)	3483882..3485246	+	1365	WP_001000978.1	MFS transporter	-
IGI33_RS16785 (3485374)	3485374..3485865	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
IGI33_RS16790 (3486033)	3486033..3486944	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T203735 WP_000227784.1 NZ_CP075477:3481941-3482483 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T203735 NZ_CP100307:3433-3720 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT203735 WP_001297137.1 NZ_CP075477:3481646-3481957 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT203735 NZ_CP100307:3707-4021 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3