Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3044569..3045212 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag IGI33_RS14665 Protein ID WP_000754566.1
Coordinates 3044569..3044985 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IGI33_RS14670 Protein ID WP_001261276.1
Coordinates 3044982..3045212 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS14635 (3039607) 3039607..3040338 + 732 WP_001295905.1 arginine ABC transporter substrate-binding protein ArtJ -
IGI33_RS14640 (3040513) 3040513..3041640 - 1128 WP_001149732.1 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC -
IGI33_RS14645 (3041681) 3041681..3042169 - 489 WP_000389260.1 YbjO family protein -
IGI33_RS14650 (3042229) 3042229..3043074 - 846 WP_001061657.1 putrescine ABC transporter permease PotI -
IGI33_RS14655 (3043071) 3043071..3043532 - 462 Protein_2871 ABC transporter permease subunit -
IGI33_RS14660 (3043535) 3043535..3044364 - 830 Protein_2872 IS3 family transposase -
IGI33_RS14665 (3044569) 3044569..3044985 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
IGI33_RS14670 (3044982) 3044982..3045212 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IGI33_RS23510 (3045169) 3045169..3045423 + 255 Protein_2875 hypothetical protein -
IGI33_RS14675 (3045411) 3045411..3045902 - 492 Protein_2876 IS5-like element ISKpn26 family transposase -
IGI33_RS14680 (3046205) 3046205..3047467 - 1263 WP_000608644.1 IS1380-like element ISEcp1 family transposase -
IGI33_RS14685 (3047655) 3047655..3048149 - 495 Protein_2878 putrescine ABC transporter permease PotH -
IGI33_RS14690 (3048159) 3048159..3049292 - 1134 WP_000996018.1 putrescine ABC transporter ATP-binding subunit PotG -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 3043502..3047467 3965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T203733 WP_000754566.1 NZ_CP075477:c3044985-3044569 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T203733 NZ_CP100305:c2788347-2787958 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT203733 WP_001261276.1 NZ_CP075477:c3045212-3044982 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT203733 NZ_CP100305:c2788611-2788354 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A387K1G3


Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References