Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2486703..2487341 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IGI33_RS11965 Protein ID WP_000813794.1
Coordinates 2487165..2487341 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IGI33_RS11960 Protein ID WP_001270286.1
Coordinates 2486703..2487119 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS11940 (2481855) 2481855..2482796 - 942 WP_001251315.1 ABC transporter permease -
IGI33_RS11945 (2482797) 2482797..2483810 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
IGI33_RS11950 (2483828) 2483828..2484973 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
IGI33_RS11955 (2485218) 2485218..2486624 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
IGI33_RS11960 (2486703) 2486703..2487119 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IGI33_RS11965 (2487165) 2487165..2487341 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IGI33_RS11970 (2487563) 2487563..2487793 + 231 WP_000494244.1 YncJ family protein -
IGI33_RS11975 (2487885) 2487885..2489846 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IGI33_RS11980 (2489919) 2489919..2490455 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IGI33_RS11985 (2490508) 2490508..2491722 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T203732 WP_000813794.1 NZ_CP075477:c2487341-2487165 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T203732 NZ_CP100305:c1950628-1950446 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT203732 WP_001270286.1 NZ_CP075477:c2487119-2486703 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT203732 NZ_CP100305:c1950353-1949949 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References