Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 295478..296278 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag IGI33_RS01430 Protein ID WP_000342449.1
Coordinates 295751..296278 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag IGI33_RS01425 Protein ID WP_001277108.1
Coordinates 295478..295744 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS01405 (291136) 291136..291804 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
IGI33_RS01410 (291797) 291797..292855 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
IGI33_RS01415 (293100) 293100..293954 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
IGI33_RS01420 (294225) 294225..295328 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IGI33_RS01425 (295478) 295478..295744 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
IGI33_RS01430 (295751) 295751..296278 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
IGI33_RS01435 (296275) 296275..296658 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
IGI33_RS01440 (297082) 297082..298191 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IGI33_RS01445 (298239) 298239..299165 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IGI33_RS01450 (299162) 299162..300439 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
IGI33_RS01455 (300436) 300436..301203 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T203716 WP_000342449.1 NZ_CP075477:295751-296278 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T203716 NZ_CP100292:2502241-2502591 [Burkholderia glumae]
ATGGTGAAGCGTGTCAAGTTCGAGCGTGGCGACATCGTGCGAGTTAGCCTGAATCCGACGATGGGAAAGGAGCAACAAGG
GGATTTTCGGCCCGCGCTCGTTCTGTCTCCCGCTGCATTCAATGCGCTCGGCGTGGCGCTAGTCGCGCCGATTACGCAAG
GCGGTGAATTTGCGCGATATGCGGGGTTCGCCGTGCCGCTCTCGGGTTCGGGCACCGAAACGCAAGGCGTCGCGCTTGTG
AACATGGTTCGCACGCTCGATCTTGAAGCGCGCGGCGCACGCAAGATTGAGCGCGCGCCGGTCGAGGTTGTCGAAGATGC
GCTCGCGCGCCTTCAAACAATTTTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT203716 WP_001277108.1 NZ_CP075477:295478-295744 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT203716 NZ_CP100292:2501996-2502247 [Burkholderia glumae]
ATGGAACTCAAGATTCAGAAATGGGGCAATAGTGCCGCCGTCCGGCTTCCTGCAGTGTTACTGGAGCAGATCGGCGCATC
GGCGGGTAGCTCGCTGAATGCCGATGTGCGGCCCGATGGCGTATTGCTTACGCCTGCTCGTCGCAAGTATTCGCTCGATG
AACTTGTCGCGCAGTGCGACGCGAATGCCCCTGAACCGGCCGACCTTGCTGCATGGGGTGAGGTCAACGCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References