Detailed information of TA system TA203714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 40658..41456
• Replicon: plasmid pNT1N28-108k
• Accession: NZ_CP075475
• Organism: Escherichia coli strain NT1N28

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: IGI29_RS23115
• Protein ID: WP_193115156.1
• Coordinates: 40935..41456 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: IGI29_RS23110
• Protein ID: WP_001711191.1
• Coordinates: 40658..40927 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI29_RS23085	36186..37526	-	1341	WP_033545989.1	DnaB-like helicase C-terminal domain-containing protein	-
IGI29_RS23090	37587..38312	-	726	WP_193115158.1	hypothetical protein	-
IGI29_RS23095	38510..39268	-	759	WP_193115157.1	DUF2971 domain-containing protein	-
IGI29_RS23100	39314..39667	-	354	WP_174563990.1	hypothetical protein	-
IGI29_RS23105	39673..40338	-	666	WP_174563949.1	AAA family ATPase	-
IGI29_RS23110	40658..40927	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
IGI29_RS23115	40935..41456	+	522	WP_193115156.1	GNAT family N-acetyltransferase	Toxin
IGI29_RS23120	41625..41876	-	252	WP_193115155.1	hypothetical protein	-
IGI29_RS23125	41879..42571	-	693	WP_033546097.1	membrane protein	-
IGI29_RS23130	42585..42908	-	324	WP_001711185.1	hypothetical protein	-
IGI29_RS23135	42983..43765	-	783	WP_193115154.1	receptor-recognizing protein	-
IGI29_RS23670	43832..44233	-	402	WP_225902027.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108720	108720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (47-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19323.15 Da
• Isoelectric Point: 8.6396

>T203714 WP_193115156.1 NZ_CP075475:40935-41456 [Escherichia coli]
VSNTTIGIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKENLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T203714 NZ_CP100291:c2842069-2841887 [Burkholderia glumae]
ATGAACAGCGCGAACCTTATCCGAACCCTGAAAGCCGACGGCTGGTATCTCGTCAATACCGTCGGCTCGCATCACCAGTT
CAAGCATGCGACCAAGCCCGGTAAGGTGACGGTGCCTCACCCGAAGAAAGACCTTCCCCTAGGCACCGTGCGCAGCATCC
TGAAACAAGCCGGTCTGAAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT203714 WP_001711191.1 NZ_CP075475:40658-40927 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT203714 NZ_CP100291:c2841860-2841465 [Burkholderia glumae]
ATGCTCTATCCGCTCTATGTCCACGTCGGCGACGACAAGCACGCACATGGCGTCACTTTTCCCGACTTCCCCGGCTGCCA
CGCTGCGGCCGACTCCTGGGACGAACTGCCCGCAGCCGTGCAGGAGGCCGCCCAGGCCCATTTCGAGGGCGACGACAGCC
CGATTCCCGAGCCTTCCGCGCTCGAAGATTTGACGCGCGATCCGGAATACACCGGCGGCGTCTGGATGATCTTCGATATC
GACCTGTCGAAGATCAACACGAAAGCCGTGCGCTTCAACGTCAGCATGTCCGAGCGGCTGCTCCAGAAGATCGACGCCAA
GGCGCGCGCGCGCAAACTCTCGCGATCGGCCTTCCTTGCGATTGCGGCAGAACACGAAATGGAAGCTGACGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2