Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4173892..4174389 Replicon chromosome
Accession NZ_CP075474
Organism Escherichia coli strain NT1N28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI29_RS20665 Protein ID WP_001173343.1
Coordinates 4173892..4174065 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI29_RS20670 Protein ID WP_000398619.1
Coordinates 4174093..4174389 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI29_RS20655 (4170562) 4170562..4172019 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
IGI29_RS20660 (4172256) 4172256..4173773 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI29_RS20665 (4173892) 4173892..4174065 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI29_RS20670 (4174093) 4174093..4174389 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI29_RS20675 (4174616) 4174616..4174888 - 273 WP_073544413.1 GNAT family N-acetyltransferase -
IGI29_RS20680 (4174900) 4174900..4175130 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IGI29_RS20685 (4175311) 4175311..4176942 + 1632 WP_001216477.1 sensor histidine kinase -
IGI29_RS20690 (4176939) 4176939..4177658 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4154149..4174389 20240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203712 WP_001173343.1 NZ_CP075474:c4174065-4173892 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203712 NZ_CP100291:2221837-2222019 [Burkholderia glumae]
ATGAACAGCGCGAACCTTATCCGAACCCTGAAAGCCGACGGCTGGTATCTCGTCAACACCGTCGGCTCGCATCACCAGTT
CAAGCATGCGACCAAGCCCGGTAAGGTGACGGTGCCTCACCCGAAGAAAGACCTTCCCCTCGGCACCGTGCGCAGCATCC
TGAAACAAGCCGGTCTGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203712 WP_000398619.1 NZ_CP075474:c4174389-4174093 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203712 NZ_CP100291:2222046-2222441 [Burkholderia glumae]
ATGCTCTATCCGCTCTATGTCCACGTCGGTGACGACAAGCACGCCCACGGTGTCACCTTCCCCGACTTTCCCGGCTGCCA
CGCAGCGGCCGACTCCTGGGACGAACTGCCTGCAGCCGTGCAGGAGGCCGCGCAGGCTCATTTCGAGGGCGACGACAGCC
CGATTCCCGAGCCTTCCGCGCTCGAAGATTTGACGCGCGATCCGGAATACACCGGCGGCGTCTGGATGATCTTCGATATC
GACCTGTCGAAGATCAACACGAAGGCCGTGCGCTTCAACGTCAGCATGTCCGAACGCCTGCTGCAGCAGATCGACGCAAC
CGCGCGGGAGCGTAAGCTGTCGCGCTCGGCGTTCCTCGCGCTCGCCGCTGAACATGAGATGCGGGCCGGCGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References