Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3424586..3425204 Replicon chromosome
Accession NZ_CP075474
Organism Escherichia coli strain NT1N28

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IGI29_RS17150 Protein ID WP_001291435.1
Coordinates 3424986..3425204 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IGI29_RS17145 Protein ID WP_000344800.1
Coordinates 3424586..3424960 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI29_RS17135 (3419675) 3419675..3420868 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IGI29_RS17140 (3420891) 3420891..3424040 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
IGI29_RS17145 (3424586) 3424586..3424960 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IGI29_RS17150 (3424986) 3424986..3425204 + 219 WP_001291435.1 HHA domain-containing protein Toxin
IGI29_RS17155 (3425376) 3425376..3425927 + 552 WP_000102564.1 maltose O-acetyltransferase -
IGI29_RS17160 (3426043) 3426043..3426513 + 471 WP_000136192.1 YlaC family protein -
IGI29_RS17165 (3426677) 3426677..3428227 + 1551 WP_023486216.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IGI29_RS17170 (3428269) 3428269..3428622 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IGI29_RS17180 (3429001) 3429001..3429312 + 312 WP_000409911.1 MGMT family protein -
IGI29_RS17185 (3429343) 3429343..3429915 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203710 WP_001291435.1 NZ_CP075474:3424986-3425204 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T203710 NZ_CP100289:c57191-56697 [Burkholderia glumae]
ATGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CAAGTGGCTCAAACGTCGTGCATTGGCCAATCAGCAAAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGGG
TGGGGGCGTACTACGCACTCGCGTCCGGCGTGGTTACGCCCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCCGTGGTTGTGCTCGGGCGTCTTGCCGTTGATCGCGCCCATCAGGGCAATGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGTTTGCGTGTGCTGCACGCCGCAGCGGCCATCGGAATTCGCGGCCTGATTGTCCATGCGCTCACGGATTCGG
CCAAGGCGTTTTACGAGCGGGTGGGCTTTGAGGCGTCGCCAATCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTT
GAACACGCCTTATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT203710 WP_000344800.1 NZ_CP075474:3424586-3424960 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT203710 NZ_CP100289:c57469-57188 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACCCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATCGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTCTTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGCCTGGATGCGCCCGCCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References