Detailed information of TA system TA020371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4359577..4360208
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ECs_4357
• Protein ID: NP_312384.1
• Coordinates: 4359933..4360208 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECs_4356
• Protein ID: NP_312383.1
• Coordinates: 4359577..4359936 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_4350	4354689..4355162	+	474	NP_312377.1	phosphotransferase system enzyme IIA	-
ECs_4351	4355159..4355440	+	282	NP_312378.1	phosphotransferase system enzyme IIB	-
ECs_4352	4355517..4356875	+	1359	NP_312379.1	phosphotransferase system enzyme IIC	-
ECs_4353	4356868..4358376	+	1509	NP_312380.1	L-fuculokinase	-
ECs_4354	4358366..4358635	+	270	NP_312381.1	phosphotransferase system HPr enzyme	-
ECs_4355	4358667..4359527	+	861	NP_312382.1	aldolase	-
ECs_4356	4359577..4359936	-	360	NP_312383.1	DNA repair protein	Antitoxin
ECs_4357	4359933..4360208	-	276	NP_312384.1	HicA-like protein	Toxin
ECs_4358	4360281..4361405	-	1125	NP_312385.2	ABC transporter permease	-
ECs_4359	4361405..4364140	-	2736	NP_312386.2	ribosome-associated ATPase: ATP-binding protein/ATP-binding membrane protein	-
ECs_4360	4364137..4365204	-	1068	NP_312387.1	membrane fusion protein of efflux pump	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10165.92 Da
• Isoelectric Point: 10.7076

>T20371 NP_312384.1 NC_002695:c4360208-4359933 [Escherichia coli O157:H7 str. Sakai]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T20371 NC_002695:c4360208-4359933 [Escherichia coli O157:H7 str. Sakai]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT20371 NP_312383.1 NC_002695:c4359936-4359577 [Escherichia coli O157:H7 str. Sakai]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT20371 NC_002695:c4359936-4359577 [Escherichia coli O157:H7 str. Sakai]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA