Detailed information of TA system TA020370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4316977..4317777
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ECs_4307
• Protein ID: NP_312334.1
• Coordinates: 4316977..4317504 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: ECs_4308
• Protein ID: NP_312335.1
• Coordinates: 4317511..4317777 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_4302	4312052..4312819	-	768	NP_312329.1	branched-chain amino acid ABC transporter ATPase	-
ECs_4303	4312816..4314093	-	1278	NP_312330.1	branched-chain amino acid ABC transporter permease	-
ECs_4304	4314090..4315016	-	927	NP_312331.1	branched-chain amino acid ABC transporter permease	-
ECs_4305	4315064..4316173	-	1110	NP_312332.1	leucine transporter subunit	-
ECs_4306	4316597..4316980	+	384	NP_312333.1	PanD autocleavage accelerator	-
ECs_4307	4316977..4317504	-	528	NP_312334.1	acetyltransferase	Toxin
ECs_4308	4317511..4317777	-	267	NP_312335.1	toxin-antitoxin system antitoxin component	Antitoxin
ECs_4309	4317927..4319030	-	1104	NP_312336.2	leucine transporter subunit	-
ECs_4310	4319301..4320155	-	855	NP_312337.1	RNA polymerase sigma 32 factor RpoH	-
ECs_4311	4320400..4321458	-	1059	NP_312338.1	cell division protein FtsX	-
ECs_4312	4321451..4322119	-	669	NP_312339.1	cell division ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T20370 NP_312334.1 NC_002695:c4317504-4316977 [Escherichia coli O157:H7 str. Sakai]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T20370 NC_002695:c4317504-4316977 [Escherichia coli O157:H7 str. Sakai]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT20370 NP_312335.1 NC_002695:c4317777-4317511 [Escherichia coli O157:H7 str. Sakai]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT20370 NC_002695:c4317777-4317511 [Escherichia coli O157:H7 str. Sakai]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA