Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 59306..59828 Replicon plasmid pNT1N28-tetX4
Accession NZ_CP075473
Organism Escherichia coli strain NT1N28

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI29_RS00320 Protein ID WP_000220560.1
Coordinates 59306..59587 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI29_RS00325 Protein ID WP_000121743.1
Coordinates 59577..59828 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI29_RS00295 56585..56782 + 198 WP_000843497.1 DUF2933 domain-containing protein -
IGI29_RS00300 56816..57106 - 291 WP_073503549.1 peptidoglycan DD-metalloendopeptidase family protein -
IGI29_RS00305 57126..58094 + 969 WP_077783444.1 IS5 family transposase -
IGI29_RS00310 58131..58388 - 258 WP_105278499.1 hypothetical protein -
IGI29_RS00320 59306..59587 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI29_RS00325 59577..59828 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI29_RS00330 61062..61898 + 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI29_RS00335 61938..62384 + 447 Protein_67 hypothetical protein -
IGI29_RS00340 62521..62877 + 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI29_RS00345 62874..63851 - 978 WP_024245156.1 hypothetical protein -
IGI29_RS00350 63876..64157 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA5 / sul3 / mef(B) / qnrS1 / tet(M) / blaTEM-1B / tet(A) / floR / tet(X4) - 1..148421 148421
- flank IS/Tn - - 57171..58094 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203692 WP_000220560.1 NZ_CP075473:c59587-59306 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203692 NZ_CP100270:c131793-131491 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203692 WP_000121743.1 NZ_CP075473:c59828-59577 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203692 NZ_CP100270:c132028-131783 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References