Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4148320..4148817 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI24_RS21115 Protein ID WP_001173343.1
Coordinates 4148320..4148493 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI24_RS21120 Protein ID WP_000398619.1
Coordinates 4148521..4148817 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS21105 (4144990) 4144990..4146447 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IGI24_RS21110 (4146684) 4146684..4148201 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI24_RS21115 (4148320) 4148320..4148493 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI24_RS21120 (4148521) 4148521..4148817 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI24_RS21125 (4149044) 4149044..4149316 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IGI24_RS21130 (4149328) 4149328..4149558 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
IGI24_RS21135 (4149739) 4149739..4151370 + 1632 WP_001216477.1 sensor histidine kinase -
IGI24_RS21140 (4151367) 4151367..4152086 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203691 WP_001173343.1 NZ_CP075472:c4148493-4148320 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203691 NZ_CP100270:c125216-124866 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203691 WP_000398619.1 NZ_CP075472:c4148817-4148521 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203691 NZ_CP100270:c125461-125210 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References