Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1062440..1063281 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag IGI24_RS05845 Protein ID WP_000854822.1
Coordinates 1062440..1062823 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M0CWL2
Locus tag IGI24_RS05850 Protein ID WP_053271974.1
Coordinates 1062913..1063281 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS05820 (1057824) 1057824..1060076 - 2253 Protein_1018 alpha-amylase family glycosyl hydrolase -
IGI24_RS05830 (1060810) 1060810..1061655 - 846 WP_001280435.1 DUF4942 domain-containing protein -
IGI24_RS05835 (1061752) 1061752..1061949 - 198 WP_000839263.1 DUF957 domain-containing protein -
IGI24_RS05840 (1061961) 1061961..1062449 - 489 WP_001177592.1 DUF5983 family protein -
IGI24_RS05845 (1062440) 1062440..1062823 - 384 WP_000854822.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
IGI24_RS05850 (1062913) 1062913..1063281 - 369 WP_053271974.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IGI24_RS05855 (1063361) 1063361..1063582 - 222 WP_000692312.1 DUF987 domain-containing protein -
IGI24_RS05860 (1063651) 1063651..1064127 - 477 WP_001186747.1 RadC family protein -
IGI24_RS05865 (1064142) 1064142..1064627 - 486 WP_000214317.1 antirestriction protein -
IGI24_RS05870 (1064718) 1064718..1065536 - 819 WP_001234702.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1059039..1073027 13988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T203673 WP_000854822.1 NZ_CP075472:c1062823-1062440 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T203673 NZ_CP100247:c129107-128757 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13679.41 Da        Isoelectric Point: 6.4758

>AT203673 WP_053271974.1 NZ_CP075472:c1063281-1062913 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT203673 NZ_CP100247:c129352-129101 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CWL2

References