Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 846851..847505 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IGI24_RS04800 Protein ID WP_000244781.1
Coordinates 847098..847505 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IGI24_RS04795 Protein ID WP_000354046.1
Coordinates 846851..847117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS04770 (842020) 842020..842763 + 744 WP_032298494.1 SDR family oxidoreductase -
IGI24_RS04775 (842820) 842820..844253 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IGI24_RS04780 (844298) 844298..844609 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IGI24_RS04785 (844773) 844773..845432 + 660 WP_000250274.1 hemolysin III family protein -
IGI24_RS04790 (845628) 845628..846608 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
IGI24_RS04795 (846851) 846851..847117 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IGI24_RS04800 (847098) 847098..847505 + 408 WP_000244781.1 protein YgfX Toxin
IGI24_RS04805 (847545) 847545..848066 - 522 WP_001055874.1 flavodoxin FldB -
IGI24_RS04810 (848178) 848178..849074 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IGI24_RS04815 (849099) 849099..849809 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IGI24_RS04820 (849815) 849815..851548 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T203671 WP_000244781.1 NZ_CP075472:847098-847505 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203671 NZ_CP100246:107097-107591 [Burkholderia glumae]
ATGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CAAGTGGCTCAAACGTCGTGCATTGGCCAATCAGCGAAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGGG
TGGGGGCGTACTACGCACTCGCGTCCGGCGTGGTTACGCCCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCCGTGGTTGTGCTCGGGCGTCTTGCCGTTGATCGCGCCCATCAGGGCAATGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGTTTGCGTGTGCTGCACGCCGCAGCGGCCATCGGAATTCGCGGCCTGATTGTCCATGCGCTCACGGATTCGG
CCAAGGCGTTTTACGAGCGGGTGGGCTTTGAGGCGTCGCCAATCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTT
GAACACGCCTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203671 WP_000354046.1 NZ_CP075472:846851-847117 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203671 NZ_CP100246:106819-107100 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACCCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATCGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTCTTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGCCTGGATGCGCCCGCCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References