Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 64279..64801 Replicon plasmid pNT1W22-tetX4
Accession NZ_CP075470
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI32_RS23460 Protein ID WP_000220560.1
Coordinates 64520..64801 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI32_RS23455 Protein ID WP_000121743.1
Coordinates 64279..64530 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS23430 59950..60231 + 282 WP_001185482.1 hypothetical protein -
IGI32_RS23435 60256..61233 + 978 WP_024245156.1 hypothetical protein -
IGI32_RS23440 61230..61586 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI32_RS23445 61723..62169 - 447 Protein_74 hypothetical protein -
IGI32_RS23450 62209..63045 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI32_RS23455 64279..64530 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI32_RS23460 64520..64801 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI32_RS23465 64876..65573 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
IGI32_RS23470 65719..65976 + 258 WP_105278499.1 hypothetical protein -
IGI32_RS23475 66013..66981 - 969 WP_077783444.1 IS5 family transposase -
IGI32_RS23480 67001..67291 + 291 WP_073503549.1 peptidoglycan DD-metalloendopeptidase family protein -
IGI32_RS23485 67325..67522 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mef(B) / sul3 / dfrA5 / tet(X4) / floR / tet(A) / blaTEM-1B / tet(M) / qnrS1 - 1..101476 101476
- flank IS/Tn - - 66013..66981 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203666 WP_000220560.1 NZ_CP075470:64520-64801 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203666 NZ_CP100240:5823-6356 [Burkholderia glumae]
ATGATCCTCACGCCGTTGCAGGACATCACGGCCTATCGCATGCATGTGCCCAAGTGGGCCGTCGCGCCCACCAGCGGCGC
TGGCGCGGCCAAGGCCGGGGGGCGGGCCAACCGGATCGGTACGCCAGCGTTGTACCTGGCGCTGGAGGCGGCCACCGCGG
TGGCCGAGTACCAGCAGCTGTCGCCCCTGTTGCCGCCGGGCACGTTGGTGAGCTATCGCGTCACGGCCACGCCCGTGGTC
GATTTCACCGGCGGCTACCGTGTGGATCTCTGGCCGCCCCTCTGGGAGTCGTTCTTCTGCGACTGGCGGCATGACTGGTT
CAACGCGCGCATTGAGCCGCCCAGCTGGGTACTCGGCGACGAGGCAATCGCCTCCGGCGCCAAGGGCATCCTGTTTCGCT
CGACGCAGGCCGCCGGCGGCACGAACTTGGTGCTGTTCGTCGACCAGTTGGGGGAGGGCGATCGCGTGGATGTTCACGAC
CCGGCCGGCGCCTTGCCGCGCGATCAAGCGTCGTGGGTTGACTCGCCCGAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203666 WP_000121743.1 NZ_CP075470:64279-64530 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203666 NZ_CP100240:5410-5826 [Burkholderia glumae]
ATGAGCACTCTCGCAGCAGCGCGAGCCCCCGCCGGCGGTCCGGCCCTGGCAAACTTTGACGCGTTTCTCAGCGCGCTACG
CGAGCCGGACACGGCCGCTCCCGTGCTGTCGGCTCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGACGCTCGCGG
ACCAGGCTCACGTCCATCGCAACACCGTCGCGCGCGCGCCGCAGTCAGCCAGCGTCCAGCAGTTTCTGCGCGAGGCGATT
CGCGTGATCCGAGCCGCGACGGATCTGTCGGGCGACGTTCAACGCGCGCTGTTCTGGTATCGCAACGAGCCTCTGGCCGT
CTTCGATTACCAGACGGCCGAAACGCTGGTGTCGGCAGGGCGCGCCGATGACGTTGTGCGCTATGTGGCATCGCTCGAGG
CGGGCGCCGCCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References