Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4283201..4283698 Replicon chromosome
Accession NZ_CP075469
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI32_RS20890 Protein ID WP_001173343.1
Coordinates 4283201..4283374 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI32_RS20895 Protein ID WP_000398619.1
Coordinates 4283402..4283698 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS20880 (4279866) 4279866..4281323 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IGI32_RS20885 (4281560) 4281560..4283077 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI32_RS20890 (4283201) 4283201..4283374 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI32_RS20895 (4283402) 4283402..4283698 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI32_RS20900 (4283925) 4283925..4284197 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IGI32_RS20905 (4284209) 4284209..4284439 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IGI32_RS20910 (4284620) 4284620..4286251 + 1632 WP_001565376.1 sensor histidine kinase -
IGI32_RS20915 (4286248) 4286248..4286967 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203665 WP_001173343.1 NZ_CP075469:c4283374-4283201 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203665 NZ_CP100239:c1706294-1705797 [Burkholderia glumae]
GTGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGCCGTGCACTGGCCAATCAGCGTAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGAG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCGGTGGTTGTGCTCGGGCGTCTCGCCGTCGATCGCGCCCATCAGGGCATTGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGCTTGCGTGTGTTGCACGCCGCAGCGGCCATCGGGATTCGCGGCCTGATCGTGCATGCACTCTCGGACTCGG
CCAAGGCGTTCTATGAGCGGGTCGGCTTTGAGGCGTCGCCGCTCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTC
GAGCACGCCTTGTCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203665 WP_000398619.1 NZ_CP075469:c4283698-4283402 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203665 NZ_CP100239:c1706572-1706291 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACTCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTTCTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGTTTGGACGAGCCCGTCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References