Detailed information of TA system TA020366

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3778692..3779346
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: ECs_3768
• Protein ID: NP_311795.1
• Coordinates: 3778692..3779099 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: ECs_3769
• Protein ID: NP_311796.1
• Coordinates: 3779080..3779346 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_3764	3774649..3776382	-	1734	NP_311791.1	single-stranded-DNA-specific exonuclease RecJ	-
ECs_3765	3776388..3777098	-	711	NP_311792.1	thiol:disulfide interchange protein DsbC	-
ECs_3766	3777123..3778019	-	897	NP_311793.1	tyrosine recombinase XerD	-
ECs_3767	3778131..3778652	+	522	NP_311794.1	flavodoxin 2	-
ECs_3768	3778692..3779099	-	408	NP_311795.1	toxin of CptAB toxin-antitoxin pair	Toxin
ECs_3769	3779080..3779346	-	267	NP_311796.1	flavinator of succinate dehydrogenase	Antitoxin
ECs_3770	3779589..3780569	+	981	NP_311797.1	iron-sulfur cluster repair protein	-
ECs_3771	3780646..3781305	-	660	NP_311798.1	hemolysin III family HylIII inner membrane protein	-
ECs_3772	3781469..3781780	-	312	NP_311799.1	hypothetical protein	-
ECs_3773	3781825..3783258	+	1434	NP_311800.2	6-phospho-beta-glucosidase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T20366 NP_311795.1 NC_002695:c3779099-3778692 [Escherichia coli O157:H7 str. Sakai]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T20366 NC_002695:c3779099-3778692 [Escherichia coli O157:H7 str. Sakai]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT20366 NP_311796.1 NC_002695:c3779346-3779080 [Escherichia coli O157:H7 str. Sakai]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT20366 NC_002695:c3779346-3779080 [Escherichia coli O157:H7 str. Sakai]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA