Detailed information of TA system TA020365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3633282..3633865
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ECs_3642
• Protein ID: NP_311669.1
• Coordinates: 3633282..3633617 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ECs_3643
• Protein ID: NP_311670.1
• Coordinates: 3633617..3633865 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_3639	3629169..3630467	-	1299	NP_311666.1	enolase	-
ECs_3640	3630555..3632192	-	1638	NP_311667.1	CTP synthetase	-
ECs_3641	3632420..3633211	-	792	NP_311668.1	nucleoside triphosphate pyrophosphohydrolase	-
ECs_3642	3633282..3633617	-	336	NP_311669.1	mRNA interferase toxin	Toxin
ECs_3643	3633617..3633865	-	249	NP_311670.1	antitoxin of toxin-antitoxin system	Antitoxin
ECs_3644	3633943..3636177	-	2235	NP_311671.1	(p)ppGpp synthetase RelA	-
ECs_3645	3636225..3637526	-	1302	NP_311672.2	23S rRNA m(5)U1939 methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T20365 NP_311669.1 NC_002695:c3633617-3633282 [Escherichia coli O157:H7 str. Sakai]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T20365 NC_002695:c3633617-3633282 [Escherichia coli O157:H7 str. Sakai]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT20365 NP_311670.1 NC_002695:c3633865-3633617 [Escherichia coli O157:H7 str. Sakai]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT20365 NC_002695:c3633865-3633617 [Escherichia coli O157:H7 str. Sakai]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA