Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 19806..20328 Replicon plasmid pNT1N34-tetX4
Accession NZ_CP075467
Organism Escherichia coli strain NT1N34

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI31_RS22630 Protein ID WP_000220560.1
Coordinates 20047..20328 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI31_RS22625 Protein ID WP_000121743.1
Coordinates 19806..20057 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI31_RS22600 15477..15758 + 282 WP_001185482.1 hypothetical protein -
IGI31_RS22605 15783..16760 + 978 WP_024245156.1 hypothetical protein -
IGI31_RS22610 16757..17113 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI31_RS22615 17250..17696 - 447 Protein_22 hypothetical protein -
IGI31_RS22620 17736..18572 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI31_RS22625 19806..20057 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI31_RS22630 20047..20328 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI31_RS22635 20403..21100 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
IGI31_RS22640 21315..22160 + 846 WP_164538741.1 macrolide hydrolase EstT -
IGI31_RS22645 22150..23307 + 1158 WP_094309310.1 tetracycline-inactivating monooxygenase Tet(X4) -
IGI31_RS22650 23646..25139 + 1494 WP_001120888.1 IS91-like element ISVsa3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / tet(M) / qnrS1 / tet(X4) / dfrA5 / sul3 / mef(B) / floR / tet(A) - 1..103081 103081


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203644 WP_000220560.1 NZ_CP075467:20047-20328 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203644 NZ_CP100212:5823-6356 [Burkholderia glumae]
ATGATCCTCACGCCGTTGCAGGACATCACGGCCTATCGCATGCATGTGCCCAAGTGGGCCGTCGCGCCCACCAGCGGCGC
TGGCGCGGCCAAGGCCGGGGGGCGGGCCAACCGGATCGGTACGCCAGCGTTGTACCTGGCGCTGGAGGCGGCCACCGCGG
TGGCCGAGTACCAGCAGCTGTCGCCCCTGTTGCCGCCGGGCACGTTGGTGAGCTATCGCGTCACGGCCACGCCCGTGGTC
GATTTCACCGGCGGCTACCGTGTGGATCTCTGGCCGCCCCTCTGGGAGTCGTTCTTCTGCGACTGGCGGCATGACTGGTT
CAACGCGCGCATTGAGCCGCCCAGCTGGGTACTCGGCGACGAGGCAATCGCCTCCGGCGCCAAGGGCATCCTGTTTCGCT
CGACGCAGGCCGCCGGCGGCACGAACTTGGTGCTGTTCGTCGACCAGTTGGGGGAGGGCGATCGCGTGGATGTTCACGAC
CCGGCCGGCGCCTTGCCGCGCGATCAAGCGTCGTGGGTTGACTCGCCCGAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203644 WP_000121743.1 NZ_CP075467:19806-20057 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203644 NZ_CP100212:5410-5826 [Burkholderia glumae]
ATGAGCACTCTCGCAGCAGCGCGAGCCCCCGCCGGCGGTCCGGCCCTGGCAAACTTTGACGCGTTTCTCAGCGCGCTACG
CGAGCCGGACACGGCCGCTCCCGTGCTGTCGGCTCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGACGCTCGCGG
ACCAGGCTCACGTCCATCGCAACACCGTCGCGCGCGCGCCGCAGTCAGCCAGCGTCCAGCAGTTTCTGCGCGAGGCGATT
CGCGTGATCCGAGCCGCGACGGATCTGTCGGGCGACGTTCAACGCGCGCTGTTCTGGTATCGCAACGAGCCTCTGGCCGT
CTTCGATTACCAGACGGCCGAAACGCTGGTGTCGGCAGGGCGCGCCGATGACGTTGTGCGCTATGTGGCATCGCTCGAGG
CGGGCGCCGCCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References