Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3392961..3393579 Replicon chromosome
Accession NZ_CP075465
Organism Escherichia coli strain NT1N34

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IGI31_RS16325 Protein ID WP_001291435.1
Coordinates 3393361..3393579 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IGI31_RS16320 Protein ID WP_000344800.1
Coordinates 3392961..3393335 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI31_RS16310 (3388050) 3388050..3389243 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IGI31_RS16315 (3389266) 3389266..3392415 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
IGI31_RS16320 (3392961) 3392961..3393335 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IGI31_RS16325 (3393361) 3393361..3393579 + 219 WP_001291435.1 HHA domain-containing protein Toxin
IGI31_RS16330 (3393751) 3393751..3394302 + 552 WP_000102564.1 maltose O-acetyltransferase -
IGI31_RS16335 (3394418) 3394418..3394888 + 471 WP_000136192.1 YlaC family protein -
IGI31_RS16340 (3395052) 3395052..3396602 + 1551 WP_088537107.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IGI31_RS16345 (3396644) 3396644..3396997 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IGI31_RS16355 (3397376) 3397376..3397687 + 312 WP_000409911.1 MGMT family protein -
IGI31_RS16360 (3397718) 3397718..3398290 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203637 WP_001291435.1 NZ_CP075465:3393361-3393579 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T203637 NZ_CP100205:c125216-124866 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT203637 WP_000344800.1 NZ_CP075465:3392961-3393335 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT203637 NZ_CP100205:c125461-125210 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References