Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 60347..60869 Replicon plasmid pNT1F10-tetX4
Accession NZ_CP075463
Organism Escherichia coli strain NT1F10

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IGI19_RS23705 Protein ID WP_000220560.1
Coordinates 60588..60869 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IGI19_RS23700 Protein ID WP_000121743.1
Coordinates 60347..60598 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI19_RS23675 56018..56299 + 282 WP_001185482.1 hypothetical protein -
IGI19_RS23680 56324..57301 + 978 WP_024245156.1 hypothetical protein -
IGI19_RS23685 57298..57654 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
IGI19_RS23690 57791..58237 - 447 Protein_72 hypothetical protein -
IGI19_RS23695 58277..59113 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
IGI19_RS23700 60347..60598 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IGI19_RS23705 60588..60869 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IGI19_RS23710 60944..61641 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
IGI19_RS23715 61787..62044 + 258 WP_105278499.1 hypothetical protein -
IGI19_RS23720 62081..63049 - 969 WP_077783444.1 IS5 family transposase -
IGI19_RS23725 63069..63359 + 291 WP_073503549.1 peptidoglycan DD-metalloendopeptidase family protein -
IGI19_RS23730 63393..63590 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mef(B) / sul3 / dfrA5 / tet(X4) / floR / tet(A) / blaTEM-1B / tet(M) / qnrS1 - 1..97500 97500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T203615 WP_000220560.1 NZ_CP075463:60588-60869 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T203615 NZ_CP100182:2023171-2023356 [Burkholderia glumae]
ATGAAGTACAGCGAGTTCCGCAAGTGGCTGAAGAAACAGGGTGCTGAGTTCGAAAAGCACAAATCTGGTTCAAGCCACTT
CCGGGTTACTTTGAACGGCAAGACCACCATCTTCCCGGACCACGGCAGCAAGGAAATCGGTACCGGGTTGGTCGAGGCGA
TCAAGAAGCAACTCGGAATCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT203615 WP_000121743.1 NZ_CP075463:60347-60598 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT203615 NZ_CP100182:2023427-2023846 [Burkholderia glumae]
ATGCTGTCGTATCCCGTCACCCTGACGCCGGACTCGAACGGTACTTTGCTGGTCACGTTCCCGGACGTTCCGGAAGCCAT
CTCTGTTGGAGAGAACGTTGAGGACGCGCTCGCTCAAGGGCTTGATGCGCTCGAAGCAGCCTTTGAGATCTACTTCAACG
AGAAGCGGCTTATCCCCGCTCCATCACAAGCGGCGCCTGGGCAACACGTCGTTACCCTGCCCGTTCTCGTGGCATCAAAG
GTCTTGCTGGCAAACGAAGCCATCGAACAGAAAGTTCGAAAGGCCGAGCTTGCACGCCGGCTCAAGGTCGCCCCGGTGCA
GGTCGATCGTCTATTCAAGTTCAGCCATGGCTCCAAGATCGAGATGATCGAGGCTGCCCTTGGCGTGCTTGGAAAGCGCC
TAGAGATCAAGGCTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References