Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4233950..4234447 Replicon chromosome
Accession NZ_CP075462
Organism Escherichia coli strain NT1F10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI19_RS21145 Protein ID WP_001173343.1
Coordinates 4233950..4234123 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI19_RS21150 Protein ID WP_000398619.1
Coordinates 4234151..4234447 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI19_RS21135 (4230615) 4230615..4232072 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IGI19_RS21140 (4232309) 4232309..4233826 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI19_RS21145 (4233950) 4233950..4234123 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI19_RS21150 (4234151) 4234151..4234447 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI19_RS21155 (4234674) 4234674..4234946 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IGI19_RS21160 (4234958) 4234958..4235188 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IGI19_RS21165 (4235369) 4235369..4237000 + 1632 WP_001565376.1 sensor histidine kinase -
IGI19_RS21170 (4236997) 4236997..4237716 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203614 WP_001173343.1 NZ_CP075462:c4234123-4233950 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203614 NZ_CP100182:c1705048-1704551 [Burkholderia glumae]
GTGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGCCGTGCACTGGCCAATCAGCGTAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGAG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCGGTGGTTGTGCTCGGGCGTCTCGCCGTCGATCGCGCCCATCAGGGCATTGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGCTTGCGTGTGTTGCACGCCGCAGCGGCCATCGGGATTCGCGGCCTGATCGTGCATGCACTCTCGGACTCGG
CCAAGGCGTTCTATGAGCGGGTCGGCTTTGAGGCGTCGCCGCTCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTC
GAGCACGCCTTGTCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203614 WP_000398619.1 NZ_CP075462:c4234447-4234151 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203614 NZ_CP100182:c1705326-1705045 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACTCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTTCTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGTTTGGACGAGCCCGTCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References