Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3439872..3440490 Replicon chromosome
Accession NZ_CP075462
Organism Escherichia coli strain NT1F10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IGI19_RS17175 Protein ID WP_001291435.1
Coordinates 3440272..3440490 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IGI19_RS17170 Protein ID WP_000344800.1
Coordinates 3439872..3440246 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI19_RS17160 (3434961) 3434961..3436154 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IGI19_RS17165 (3436177) 3436177..3439326 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
IGI19_RS17170 (3439872) 3439872..3440246 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IGI19_RS17175 (3440272) 3440272..3440490 + 219 WP_001291435.1 HHA domain-containing protein Toxin
IGI19_RS17180 (3440662) 3440662..3441213 + 552 WP_000102564.1 maltose O-acetyltransferase -
IGI19_RS17185 (3441329) 3441329..3441799 + 471 WP_000136192.1 YlaC family protein -
IGI19_RS17190 (3441963) 3441963..3443513 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IGI19_RS17195 (3443555) 3443555..3443862 - 308 Protein_3289 DUF1428 family protein -
IGI19_RS17205 (3444241) 3444241..3444552 + 312 WP_000409911.1 MGMT family protein -
IGI19_RS17210 (3444583) 3444583..3445155 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203611 WP_001291435.1 NZ_CP075462:3440272-3440490 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T203611 NZ_CP100178:c85756-85406 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT203611 WP_000344800.1 NZ_CP075462:3439872-3440246 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT203611 NZ_CP100178:c86001-85750 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References