Detailed information of TA system TA203594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 59056..59657
• Replicon: plasmid pNT1F10-94k
• Accession: NZ_CP075461
• Organism: Escherichia coli strain NT1F10

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: IGI19_RS00350
• Protein ID: WP_001216034.1
• Coordinates: 59277..59657 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: IGI19_RS00345
• Protein ID: WP_032332867.1
• Coordinates: 59056..59277 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI19_RS00325	55524..56333	-	810	WP_214479394.1	restriction endonuclease subunit S	-
IGI19_RS00330	56330..57886	-	1557	WP_214479395.1	type I restriction-modification system subunit M	-
IGI19_RS00335	58244..58495	+	252	WP_001755493.1	DNA polymerase III subunit theta	-
IGI19_RS00340	58594..58983	+	390	WP_032332866.1	S24 family peptidase	-
IGI19_RS00345	59056..59277	+	222	WP_032332867.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
IGI19_RS00350	59277..59657	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IGI19_RS00355	59662..59841	+	180	WP_001436571.1	hypothetical protein	-
IGI19_RS00360	59869..60912	+	1044	WP_001561086.1	DUF968 domain-containing protein	-
IGI19_RS00365	61001..61453	+	453	WP_001326849.1	late promoter-activating protein	-
IGI19_RS00370	61539..62732	+	1194	WP_032332868.1	hypothetical protein	-
IGI19_RS00375	62732..64216	+	1485	WP_096321054.1	terminase	-
IGI19_RS00380	64438..64557	+	120	WP_071594056.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94512	94512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T203594 WP_001216034.1 NZ_CP075461:59277-59657 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T203594 NZ_CP100157:c1694519-1694022 [Burkholderia glumae]
GTGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGCCGTGCACTGGCCAATCAGCGTAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGAG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCGGTGGTTGTGCTCGGGCGTCTCGCCGTCGATCGCGCCCATCAGGGCATTGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGCTTGCGTGTGTTGCACGCCGCAGCGGCCATCGGGATTCGCGGCCTGATCGTGCATGCACTCTCGGACTCGG
CCAAGGCGTTCTATGAGCGGGTCGGCTTTGAGGCGTCGCCGCTCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTC
GAGCACGCCTTGTCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8132.16 Da
• Isoelectric Point: 6.3159

>AT203594 WP_032332867.1 NZ_CP075461:59056-59277 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGRKPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT203594 NZ_CP100157:c1694797-1694516 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACTCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTTCTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGTTTGGACGAGCCCGTCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCGTGA

Structures

Toxin

• AlphaFold DB: V0AJ64