Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 220316..221939 Replicon plasmid pRGF20-1-tmexCD
Accession NZ_CP075455
Organism Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag KI738_RS28455 Protein ID WP_000088044.1
Coordinates 220316..221641 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag KI738_RS28460 Protein ID WP_012477377.1
Coordinates 221646..221939 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI738_RS28425 (KI738_28440) 216302..217159 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KI738_RS28430 (KI738_28445) 217175..217483 - 309 WP_001224686.1 hypothetical protein -
KI738_RS28435 (KI738_28450) 218032..218457 - 426 WP_000278471.1 hypothetical protein -
KI738_RS28440 (KI738_28455) 218710..219525 - 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
KI738_RS28445 (KI738_28460) 219538..219948 - 411 WP_000985909.1 hypothetical protein -
KI738_RS28450 (KI738_28465) 220049..220255 - 207 WP_000134171.1 hypothetical protein -
KI738_RS28455 (KI738_28470) 220316..221641 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
KI738_RS28460 (KI738_28475) 221646..221939 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
KI738_RS28465 (KI738_28480) 222364..223368 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
KI738_RS28470 (KI738_28485) 223447..223881 - 435 WP_003131969.1 mercury resistance transcriptional regulator MerR -
KI738_RS28475 (KI738_28490) 223953..224303 + 351 WP_003131974.1 mercuric ion transporter MerT -
KI738_RS28480 (KI738_28495) 224316..224591 + 276 WP_003131987.1 mercury resistance system periplasmic binding protein MerP -
KI738_RS28485 (KI738_28500) 224663..226348 + 1686 WP_003156770.1 mercury(II) reductase -
KI738_RS28490 (KI738_28505) 226366..226731 + 366 WP_000995360.1 mercury resistance co-regulator MerD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / sul1 / blaDHA-1 / qnrB4 - 1..307852 307852
- flank IS/Tn aph(6)-Id / aph(3'')-Ib - 222364..228696 6332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T203593 WP_000088044.1 NZ_CP075455:c221641-220316 [Klebsiella pneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T203593 NZ_CP100155:c123326-122925 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT203593 WP_012477377.1 NZ_CP075455:c221939-221646 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT203593 NZ_CP100155:c123562-123326 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References