Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 192258..193881 Replicon plasmid pRGF20-1-tmexCD
Accession NZ_CP075455
Organism Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag KI738_RS28305 Protein ID WP_000088044.1
Coordinates 192258..193583 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag KI738_RS28310 Protein ID WP_012477377.1
Coordinates 193588..193881 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI738_RS28275 (KI738_28290) 188244..189101 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KI738_RS28280 (KI738_28295) 189117..189425 - 309 WP_001224686.1 hypothetical protein -
KI738_RS28285 (KI738_28300) 189974..190399 - 426 WP_000278471.1 hypothetical protein -
KI738_RS28290 (KI738_28305) 190652..191467 - 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
KI738_RS28295 (KI738_28310) 191480..191890 - 411 WP_000985909.1 hypothetical protein -
KI738_RS28300 (KI738_28315) 191991..192197 - 207 WP_000134171.1 hypothetical protein -
KI738_RS28305 (KI738_28320) 192258..193583 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
KI738_RS28310 (KI738_28325) 193588..193881 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
KI738_RS28315 (KI738_28330) 194306..195310 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
KI738_RS28320 (KI738_28335) 195389..195823 - 435 WP_003131969.1 mercury resistance transcriptional regulator MerR -
KI738_RS28325 (KI738_28340) 195895..196245 + 351 WP_003131974.1 mercuric ion transporter MerT -
KI738_RS28330 (KI738_28345) 196258..196533 + 276 WP_003131987.1 mercury resistance system periplasmic binding protein MerP -
KI738_RS28335 (KI738_28350) 196605..198290 + 1686 WP_003156770.1 mercury(II) reductase -
KI738_RS28340 (KI738_28355) 198308..198673 + 366 WP_000995360.1 mercury resistance co-regulator MerD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / sul1 / blaDHA-1 / qnrB4 - 1..307852 307852
- flank IS/Tn aph(6)-Id / aph(3'')-Ib - 194306..200638 6332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T203592 WP_000088044.1 NZ_CP075455:c193583-192258 [Klebsiella pneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T203592 NZ_CP100153:5823-6356 [Burkholderia glumae]
ATGATCCTCACGCCGTTGCAGGACATCACGGCCTATCGCATGCATGTGCCCAAGTGGGCCGTCGCGCCCACCAGCGGCGC
TGGCGCGGCCAAGGCCGGGGGGCGGGCCAACCGGATCGGTACGCCAGCGTTGTACCTGGCGCTGGAGGCGGCCACCGCGG
TGGCCGAGTACCAGCAGCTGTCGCCCCTGTTGCCGCCGGGCACGTTGGTGAGCTATCGCGTCACGGCCACGCCCGTGGTC
GATTTCACCGGCGGCTACCGTGTGGATCTCTGGCCGCCCCTCTGGGAGTCGTTCTTCTGCGACTGGCGGCATGACTGGTT
CAACGCGCGCATTGAGCCGCCCAGCTGGGTACTCGGCGACGAGGCAATCGCCTCCGGCGCCAAGGGCATCCTGTTTCGCT
CGACGCAGGCCGCCGGCGGCACGAACTTGGTGCTGTTCGTCGACCAGTTGGGGGAGGGCGATCGCGTGGATGTTCACGAC
CCGGCCGGCGCCTTGCCGCGCGATCAAGCGTCGTGGGTTGACTCGCCCGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT203592 WP_012477377.1 NZ_CP075455:c193881-193588 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT203592 NZ_CP100153:5410-5826 [Burkholderia glumae]
ATGAGCACTCTCGCAGCAGCGCGAGCCCCCGCCGGCGGTCCGGCCCTGGCAAACTTTGACGCGTTTCTCAGCGCGCTACG
CGAGCCGGACACGGCCGCTCCCGTGCTGTCGGCTCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGACGCTCGCGG
ACCAGGCTCACGTCCATCGCAACACCGTCGCGCGCGCGCCGCAGTCAGCCAGCGTCCAGCAGTTTCTGCGCGAGGCGATT
CGCGTGATCCGAGCCGCGACGGATCTGTCGGGCGACGTTCAACGCGCGCTGTTCTGGTATCGCAACGAGCCTCTGGCCGT
CTTCGATTACCAGACGGCCGAAACGCTGGTGTCGGCAGGGCGCGCCGATGACGTTGTGCGCTATGTGGCATCGCTCGAGG
CGGGCGCCGCCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References