Detailed information of TA system TA203591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 104490..105400
• Replicon: plasmid pRGF20-1-tmexCD
• Accession: NZ_CP075455
• Organism: Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: KI738_RS27875
• Protein ID: WP_004181896.1
• Coordinates: 104930..105400 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: KI738_RS27870
• Protein ID: WP_004181895.1
• Coordinates: 104490..104933 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI738_RS27845 (KI738_27855)	99645..99860	-	216	Protein_110	IS200/IS605 family transposase	-
KI738_RS27850 (KI738_27860)	99862..100431	+	570	Protein_111	transposase	-
KI738_RS27855 (KI738_27865)	101123..102610	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
KI738_RS27860 (KI738_27870)	102696..103118	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KI738_RS27865 (KI738_27875)	103118..104389	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
KI738_RS27870 (KI738_27880)	104490..104933	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
KI738_RS27875 (KI738_27885)	104930..105400	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
KI738_RS27880 (KI738_27890)	105510..105770	-	261	WP_004026352.1	hypothetical protein	-
KI738_RS27885 (KI738_27895)	106493..106651	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
KI738_RS27890 (KI738_27900)	107254..108228	+	975	WP_040222313.1	hypothetical protein	-
KI738_RS27895 (KI738_27905)	108426..108803	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / sul1 / blaDHA-1 / qnrB4	-	1..307852	307852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T203591 WP_004181896.1 NZ_CP075455:104930-105400 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T203591 NZ_CP100152:c131793-131491 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT203591 WP_004181895.1 NZ_CP075455:104490-104933 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT203591 NZ_CP100152:c132028-131783 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9