Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5241288..5241913 Replicon chromosome
Accession NZ_CP075452
Organism Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag KI738_RS25505 Protein ID WP_004187928.1
Coordinates 5241288..5241671 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KI738_RS25510 Protein ID WP_004150355.1
Coordinates 5241671..5241913 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI738_RS25490 (5238654) 5238654..5239556 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KI738_RS25495 (5239553) 5239553..5240188 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KI738_RS25500 (5240185) 5240185..5241114 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KI738_RS25505 (5241288) 5241288..5241671 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
KI738_RS25510 (5241671) 5241671..5241913 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KI738_RS25515 (5242107) 5242107..5243024 + 918 WP_004187929.1 alpha/beta hydrolase -
KI738_RS25520 (5243038) 5243038..5243979 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KI738_RS25525 (5244024) 5244024..5244461 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KI738_RS25530 (5244458) 5244458..5245318 - 861 WP_004146232.1 virulence factor BrkB family protein -
KI738_RS25535 (5245312) 5245312..5245911 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T203587 WP_004187928.1 NZ_CP075452:c5241671-5241288 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203587 NZ_CP100147:5822-6355 [Burkholderia glumae]
ATGATCCTCACGCCGTTGCAGGACATCACGGCCTATCGCATGCATGTGCCCAAGTGGGCCGTCGCGCCCACCAGCGGCGC
TGGCGCGGCCAAGGCCGGGGGGCGGGCCAACCGGATCGGTACGCCAGCGTTGTACCTGGCGCTGGAGGCGGCCACCGCGG
TGGCCGAGTACCAGCAGCTGTCGCCCCTGTTGCCGCCGGGCACGTTGGTGAGCTATCGCGTCACGGCCACGCCCGTGGTC
GATTTCACCGGCGGCTACCGTGTGGATCTCTGGCCGCCCCTCTGGGAGTCGTTCTTCTGCGACTGGCGGCATGACTGGTT
CAACGCGCGCATTGAGCCGCCCAGCTGGGTACTCGGCGACGAGGCAATCGCCTCCGGCGCCAAGGGCATCCTGTTTCGCT
CGACGCAGGCCGCCGGCGGCACGAACTTGGTGCTGTTCGTCGACCAGTTGGGGGAGGGCGATCGCGTGGATGTTCACGAC
CCGGCCGGCGCCTTGCCGCGCGATCAAGCGTCGTGGGTTGACTCGCCCGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203587 WP_004150355.1 NZ_CP075452:c5241913-5241671 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203587 NZ_CP100147:5409-5825 [Burkholderia glumae]
ATGAGCACTCTCGCAGCAGCGCGAGCCCCCGCCGGCGGTCCGGCCCTGGCAAACTTTGACGCGTTTCTCAGCGCGCTACG
CGAGCCGGACACGGCCGCTCCCGTGCTGTCGGCTCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGACGCTCGCGG
ACCAGGCTCACGTCCATCGCAACACCGTCGCGCGCGCGCCGCAGTCAGCCAGCGTCCAGCAGTTTCTGCGCGAGGCGATT
CGCGTGATCCGAGCCGCGACGGATCTGTCGGGCGACGTTCAACGCGCGCTGTTCTGGTATCGCAACGAGCCTCTGGCCGT
CTTCGATTACCAGACGGCCGAAACGCTGGTGTCGGCAGGGCGCGCCGATGACGTTGTGCGCTATGTGGCATCGCTCGAGG
CGGGCGCCGCCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References