Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4037141..4037760 Replicon chromosome
Accession NZ_CP075452
Organism Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KI738_RS19785 Protein ID WP_002892050.1
Coordinates 4037542..4037760 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KI738_RS19780 Protein ID WP_002892066.1
Coordinates 4037141..4037515 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI738_RS19770 (4032293) 4032293..4033486 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KI738_RS19775 (4033509) 4033509..4036655 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KI738_RS19780 (4037141) 4037141..4037515 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KI738_RS19785 (4037542) 4037542..4037760 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KI738_RS19790 (4037923) 4037923..4038489 + 567 WP_002892030.1 maltose O-acetyltransferase -
KI738_RS19795 (4038461) 4038461..4038601 - 141 WP_004147370.1 hypothetical protein -
KI738_RS19800 (4038622) 4038622..4039092 + 471 WP_002892026.1 YlaC family protein -
KI738_RS19805 (4039067) 4039067..4040518 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KI738_RS19810 (4040619) 4040619..4041317 + 699 WP_002892021.1 GNAT family protein -
KI738_RS19815 (4041314) 4041314..4041454 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KI738_RS19820 (4041454) 4041454..4041717 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203582 WP_002892050.1 NZ_CP075452:4037542-4037760 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T203582 NZ_CP100142:11502-11852 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT203582 WP_002892066.1 NZ_CP075452:4037141-4037515 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT203582 NZ_CP100142:11257-11508 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References