Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 136302..137925 Replicon plasmid unnamed1
Accession NZ_CP075378
Organism Escherichia coli strain no107

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KIB02_RS26050 Protein ID WP_000088046.1
Coordinates 136302..137627 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KIB02_RS26055 Protein ID WP_024136327.1
Coordinates 137632..137925 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIB02_RS26020 (KIB02_26030) 132286..133143 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KIB02_RS26025 (KIB02_26035) 133159..133467 - 309 WP_001224687.1 hypothetical protein -
KIB02_RS26030 (KIB02_26040) 134016..134441 - 426 WP_001043843.1 hypothetical protein -
KIB02_RS26035 (KIB02_26045) 134695..135510 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KIB02_RS26040 (KIB02_26050) 135523..135933 - 411 WP_000985911.1 hypothetical protein -
KIB02_RS26045 (KIB02_26055) 136035..136241 - 207 WP_000134171.1 hypothetical protein -
KIB02_RS26050 (KIB02_26060) 136302..137627 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KIB02_RS26055 (KIB02_26065) 137632..137925 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KIB02_RS26060 (KIB02_26070) 138529..138921 - 393 WP_000341066.1 hypothetical protein -
KIB02_RS26065 (KIB02_26075) 139294..139635 + 342 WP_001151575.1 hypothetical protein -
KIB02_RS26070 (KIB02_26080) 139727..139918 + 192 WP_000398480.1 hypothetical protein -
KIB02_RS26075 (KIB02_26085) 139968..140249 + 282 WP_000077926.1 hypothetical protein -
KIB02_RS27095 140590..140721 - 132 WP_000374059.1 hypothetical protein -
KIB02_RS26080 (KIB02_26090) 140812..142053 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / mcr-1.1 - 1..279642 279642


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T203567 WP_000088046.1 NZ_CP075378:c137627-136302 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T203567 NZ_CP100128:2668610-2669092 [Chromobacterium sp. IIBBL 290-4]
GTGACGCGGCTGGCGTGGCGCATTGCCACGGATACGCCGGATTATTCAGCGGAGGACATGAGCGGAGAGGGCGCCAAGCG
CACGGGCGGCCGTTGGAACCGCCGCGGCGGCGCCATGCTCTACAGCGCGGAAAGCCGAGCCTTGGCCTGCCTGGAAACCC
TGGCGCATCTGGGGGCAGGCGGCTTGCCCTTGAATCGCTACCTGGTCCGGCTGGAGATTCCCGATGCGGTTTGGGCTCGC
GCAGAGCGGGTTTCTCCAGATGATTCCGCGCTGGTGGGCTGGGATGCGCTGCCGGCGGGCCGCGTCAGTCTGGATTATGG
CGATGGCTGGCTGCGCGAGTCGCGGTCCGCCCTGCTGCTGGTTCCATCCATCATCGTGGCGGAAGAGTCGAATATCCTGA
TCAATCCGGCGCATCCGGATGCCGCCGCTATTTGCGCCGTCAAAGTGCGGAAGTGGCAGTACGATGCTCGTCTGGCTGTT
TAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT203567 WP_024136327.1 NZ_CP075378:c137925-137632 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT203567 NZ_CP100128:2668092-2668613 [Chromobacterium sp. IIBBL 290-4]
ATGGCGATGCGTCAAGCACATAGGGTTTCTGATCCGCCTGTCAGGGAGTCCAGTCATCCGGAAACAGGCAGTTATTTGCT
GGAAAAACCCTCGATTACCCATTTTCTGCGGCTCTATCGGTTGTCGCCTATGGAGCGCATCGATCTGATCCTGCGCGGCG
TGCCGGCAGCGGAGGCGAAGGAAGTGGCCAAGCGTATGGATGTGGCGCAGGACGCCATGTTCAAAGTGCTGCGGCTGCCC
ATCTCTACCGTCAACAAGCGCGCGGCGCGCAATCAGGAGCTCACCATAGAGGAGAGCGAGCGGCTGGTGGGCATGGCATC
GTTGATCGGGCAGGTGGAAAGCATGATGGCGGAATCCGGCGAGGCCGAGGATTTCGACGCGGCCAAATGGCTGGGGCAAT
GGATGGAAACGCCGAATCCTGCCTTGGGTCAGCGCCGGCCGGCGGAATTCCTGTGCACGGCTGAGGGCTTCCGTCTGGTG
TCGGATGTGTTGGCTGCGATGCAGAGCGGAGCGTATCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References