Detailed information of TA system TA203566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 78239..78798
• Replicon: plasmid unnamed4
• Accession: NZ_CP075377
• Organism: Escherichia coli strain no107

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: KIB02_RS25205
• Protein ID: WP_000421259.1
• Coordinates: 78523..78798 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: KIB02_RS25200
• Protein ID: WP_001178087.1
• Coordinates: 78239..78523 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIB02_RS25180 (73888)	73888..74421	-	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-
KIB02_RS25185 (74863)	74863..75150	-	288	WP_000074849.1	conjugal transfer protein TraA	-
KIB02_RS25190 (75068)	75068..75346	-	279	WP_219065241.1	ash family protein	-
KIB02_RS25195 (76304)	76304..77335	+	1032	WP_000907852.1	plasmid replication initiator RepA	-
KIB02_RS25200 (78239)	78239..78523	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
KIB02_RS25205 (78523)	78523..78798	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIB02_RS25210 (78904)	78904..79107	+	204	Protein_87	hypothetical protein	-
KIB02_RS25215 (79377)	79377..79643	-	267	WP_000627832.1	hypothetical protein	-
KIB02_RS25220 (79643)	79643..80137	-	495	WP_015058884.1	hypothetical protein	-
KIB02_RS25225 (80193)	80193..80795	-	603	WP_000517694.1	ProQ/FINO family protein	-
KIB02_RS25230 (81241)	81241..82440	-	1200	WP_000948429.1	IS91 family transposase	-
KIB02_RS25235 (82450)	82450..82638	-	189	WP_000957857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89891	89891
	-	flank	IS/Tn	-	-	81241..82440	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T203566 WP_000421259.1 NZ_CP075377:78523-78798 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T203566 NZ_CP100128:2564752-2565030 [Chromobacterium sp. IIBBL 290-4]
ATGATCAGATCGTTTAGATGCATCGATACTCAGGCCTTATTTGCTGGTGAGTCCGTTCCTTCTTTGGCCAACATTCGGGC
AGTGGCTGAACGGAAATTGCAGATGATTGCCTCTGCCTGTACGGTGGGCTTCCTGCGCTCTCCGCCTGGCAATCGGCTGG
AAGCCTTGAAGGGTGACCGAGAGGGGCAATTCAGCATTCGCATCAACGATCAGTGGCGTATCTGTTTTCGCTTTGAAGAC
GGTGATGCGTTTGATGTTGAAATTGTTGACTACCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT203566 WP_001178087.1 NZ_CP075377:78239-78523 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT203566 NZ_CP100128:2565043-2565345 [Chromobacterium sp. IIBBL 290-4]
ATGACCATCAAGAACGGTATGCGCCCCATCCATCCGGGCGAAATTTTACGTGAAGACTATCTGACTCCGCTGAACATGAC
ACCGCATGCGTTGTCCTTGGCGCTGCATATCCCAGCATCTCGGGTAAATGATATTGTCCGCGGGCGCCGCGGCATAACAG
TTGATACCGCCATGCGGCTGGCTCGCTACTTTGGTGGCGACGCACAAAGCTGGCTGAACCTGCAGACAGTCTATGAGCTC
AAGCAAGCTGCTGGCGCGCTGGAAACAAAGATCAGCCAAGAGATTACGCCACTGACTGCGTAA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625