Detailed information of TA system TA203561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 78231..78790
• Replicon: plasmid unnamed3
• Accession: NZ_CP075376
• Organism: Escherichia coli strain no107

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: KIB02_RS24705
• Protein ID: WP_000421259.1
• Coordinates: 78515..78790 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: KIB02_RS24700
• Protein ID: WP_001178087.1
• Coordinates: 78231..78515 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIB02_RS24680 (73880)	73880..74413	-	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-
KIB02_RS24685 (74855)	74855..75142	-	288	WP_000074849.1	conjugal transfer protein TraA	-
KIB02_RS24690 (75060)	75060..75338	-	279	WP_219065241.1	ash family protein	-
KIB02_RS24695 (76296)	76296..77327	+	1032	WP_000907852.1	plasmid replication initiator RepA	-
KIB02_RS24700 (78231)	78231..78515	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
KIB02_RS24705 (78515)	78515..78790	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIB02_RS24710 (78896)	78896..79099	+	204	Protein_88	hypothetical protein	-
KIB02_RS24715 (79369)	79369..79635	-	267	WP_000627832.1	hypothetical protein	-
KIB02_RS24720 (79635)	79635..80129	-	495	WP_015058884.1	hypothetical protein	-
KIB02_RS24725 (80185)	80185..80787	-	603	WP_000517694.1	ProQ/FINO family protein	-
KIB02_RS24730 (81141)	81141..82487	+	1347	WP_001132032.1	protein YagA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88195	88195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T203561 WP_000421259.1 NZ_CP075376:78515-78790 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T203561 NZ_CP100121:69535-69810 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT203561 WP_001178087.1 NZ_CP075376:78231-78515 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT203561 NZ_CP100121:69251-69535 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625