Detailed information of TA system TA020354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2025186..2025824
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ECs_5728
• Protein ID: YP_009502626.1
• Coordinates: 2025186..2025362 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECs_2042
• Protein ID: NP_310069.3
• Coordinates: 2025387..2025824 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_2037	2020808..2021980	-	1173	NP_310065.2	BenE family inner membrane transporter	-
ECs_2038	2022072..2022608	+	537	NP_310064.3	transcriptional regulator	-
ECs_2039	2022639..2024642	+	2004	NP_310066.1	peptidase	-
ECs_2040	2024734..2024904	-	171	NP_310067.2	hypothetical protein	-
ECs_5728	2025186..2025362	+	177	YP_009502626.1	mRNA interferase toxin of the HicAB toxin-antitoxin system	Toxin
ECs_2042	2025387..2025824	+	438	NP_310069.3	antitoxin for the HicAB toxin-antitoxin system	Antitoxin
ECs_2043	2025903..2027309	+	1407	NP_310070.1	GntR family transcriptional regulator	-
ECs_2044	2027554..2028699	+	1146	NP_310071.1	ABC transporter periplasmic binding protein	-
ECs_2045	2028717..2029730	+	1014	NP_310072.1	ABC transporter ATPase	-
ECs_2046	2029731..2030672	+	942	NP_310073.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-141)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T20354 YP_009502626.1 NC_002695:2025186-2025362 [Escherichia coli O157:H7 str. Sakai]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T20354 NC_002695:2025186-2025362 [Escherichia coli O157:H7 str. Sakai]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16105.64 Da
• Isoelectric Point: 4.6511

>AT20354 NP_310069.3 NC_002695:2025387-2025824 [Escherichia coli O157:H7 str. Sakai]
MRETVEIMRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVP
LSVASKVLLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 438 bp

>AT20354 NC_002695:2025387-2025824 [Escherichia coli O157:H7 str. Sakai]
ATGCGAGAGACAGTCGAAATTATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGT
GGATATCCCTGAAGCGTTGACGCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCAT
TTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCT
TTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAAT
TGGCAAACCTAAACAAGAGATTACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAA
AGGCGCTTGGCAAAGAGTTATCGCTGGTGATGGTTTAA