Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 906623..907277 Replicon chromosome
Accession NZ_CP075374
Organism Escherichia coli strain no107

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KIB02_RS04420 Protein ID WP_000244777.1
Coordinates 906870..907277 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KIB02_RS04415 Protein ID WP_000354046.1
Coordinates 906623..906889 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIB02_RS04390 (901792) 901792..902535 + 744 WP_000951964.1 SDR family oxidoreductase -
KIB02_RS04395 (902592) 902592..904025 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
KIB02_RS04400 (904070) 904070..904381 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KIB02_RS04405 (904545) 904545..905204 + 660 WP_000250274.1 hemolysin III family protein -
KIB02_RS04410 (905400) 905400..906380 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KIB02_RS04415 (906623) 906623..906889 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KIB02_RS04420 (906870) 906870..907277 + 408 WP_000244777.1 protein YgfX Toxin
KIB02_RS04425 (907317) 907317..907838 - 522 WP_001055874.1 flavodoxin FldB -
KIB02_RS04430 (907950) 907950..908846 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KIB02_RS04435 (908871) 908871..909581 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KIB02_RS04440 (909587) 909587..911320 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T203538 WP_000244777.1 NZ_CP075374:906870-907277 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203538 NZ_CP100114:c4696393-4695860 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203538 WP_000354046.1 NZ_CP075374:906623-906889 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203538 NZ_CP100114:c4696670-4696404 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References