Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 332829..333629 Replicon chromosome
Accession NZ_CP075374
Organism Escherichia coli strain no107

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KIB02_RS01525 Protein ID WP_000342449.1
Coordinates 333102..333629 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KIB02_RS01520 Protein ID WP_001277108.1
Coordinates 332829..333095 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIB02_RS01500 (328487) 328487..329155 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KIB02_RS01505 (329148) 329148..330206 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KIB02_RS01510 (330451) 330451..331305 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KIB02_RS01515 (331576) 331576..332679 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KIB02_RS01520 (332829) 332829..333095 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KIB02_RS01525 (333102) 333102..333629 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KIB02_RS01530 (333626) 333626..334009 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
KIB02_RS01535 (334433) 334433..335542 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KIB02_RS01540 (335590) 335590..336516 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KIB02_RS01545 (336513) 336513..337790 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
KIB02_RS01550 (337787) 337787..338554 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T203536 WP_000342449.1 NZ_CP075374:333102-333629 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T203536 NZ_CP100114:c3935068-3934751 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT203536 WP_001277108.1 NZ_CP075374:332829-333095 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT203536 NZ_CP100114:c3934758-3934471 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References