Detailed information of TA system TA203530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4400634..4401415
• Replicon: chromosome
• Accession: NZ_CP075372
• Organism: Salmonella enterica strain no75

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: E8X9W8
• Locus tag: KH432_RS21520
• Protein ID: WP_000626099.1
• Coordinates: 4400634..4401125 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B5R979
• Locus tag: KH432_RS21525
• Protein ID: WP_001110452.1
• Coordinates: 4401122..4401415 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH432_RS21485 (4396629)	4396629..4397204	+	576	WP_001188509.1	transcriptional regulator	-
KH432_RS21495 (4397475)	4397475..4397552	-	78	Protein_4227	helix-turn-helix domain-containing protein	-
KH432_RS21500 (4397643)	4397643..4397975	-	333	WP_001165471.1	MerR family transcriptional regulator	-
KH432_RS21505 (4398047)	4398047..4398424	+	378	WP_000916345.1	EthD family reductase	-
KH432_RS21510 (4399457)	4399457..4399531	+	75	Protein_4230	porin family protein	-
KH432_RS21515 (4399634)	4399634..4400386	+	753	WP_000842434.1	non-specific acid phosphatase	-
KH432_RS21520 (4400634)	4400634..4401125	-	492	WP_000626099.1	GNAT family N-acetyltransferase	Toxin
KH432_RS21525 (4401122)	4401122..4401415	-	294	WP_001110452.1	DUF1778 domain-containing protein	Antitoxin
KH432_RS21530 (4401732)	4401732..4401953	+	222	WP_001576552.1	hypothetical protein	-
KH432_RS21535 (4402218)	4402218..4403093	+	876	WP_000921674.1	AraC family transcriptional regulator	-
KH432_RS21540 (4403090)	4403090..4403377	+	288	WP_001269916.1	transcriptional regulator RtsB	-
KH432_RS21545 (4403400)	4403400..4403615	+	216	WP_001595136.1	hypothetical protein	-
KH432_RS21550 (4403623)	4403623..4403892	+	270	WP_010989096.1	hypothetical protein	-
KH432_RS21555 (4404186)	4404186..4405091	-	906	WP_001268200.1	YjiK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17646.39 Da
• Isoelectric Point: 6.8437

>T203530 WP_000626099.1 NZ_CP075372:c4401125-4400634 [Salmonella enterica]
MISTPEPLHAGHILTPFCCGVDSIDNWLEQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV

Nucleotide

• Length: 492 bp

>T203530 NZ_CP100112:c6145-5840 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10977.61 Da
• Isoelectric Point: 8.6141

>AT203530 WP_001110452.1 NZ_CP075372:c4401415-4401122 [Salmonella enterica]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT203530 NZ_CP100112:c6365-6147 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 7AK7

Antitoxin

• AlphaFold DB: A0A5I1DGA4